Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 440248..440766 Replicon chromosome
Accession NZ_CP084437
Organism Staphylococcus lugdunensis strain RMLUG3

Toxin (Protein)


Gene name TsdT Uniprot ID A0A853V532
Locus tag LE166_RS02165 Protein ID WP_002492077.1
Coordinates 440554..440766 (+) Length 71 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A853V130
Locus tag LE166_RS02160 Protein ID WP_002492098.1
Coordinates 440248..440541 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE166_RS02135 (LE166_02135) 435848..436486 + 639 WP_002492090.1 thiamine phosphate synthase -
LE166_RS02140 (LE166_02140) 436576..437451 + 876 WP_002461212.1 membrane protein insertase YidC -
LE166_RS02145 (LE166_02145) 437552..437725 - 174 WP_172412323.1 hypothetical protein -
LE166_RS02150 (LE166_02150) 437835..438476 - 642 WP_002492092.1 HD domain-containing protein -
LE166_RS02155 (LE166_02155) 438598..440082 - 1485 WP_002478972.1 cardiolipin synthase -
LE166_RS02160 (LE166_02160) 440248..440541 + 294 WP_002492098.1 copper-sensing transcriptional repressor CsoR Antitoxin
LE166_RS02165 (LE166_02165) 440554..440766 + 213 WP_002492077.1 heavy metal-associated domain-containing protein Toxin
LE166_RS02170 (LE166_02170) 440857..440994 + 138 WP_002461206.1 Lmo0850 family protein -
LE166_RS02175 (LE166_02175) 441408..442598 - 1191 WP_002461205.1 FtsW/RodA/SpoVE family cell cycle protein -
LE166_RS02180 (LE166_02180) 442954..444024 + 1071 WP_002461204.1 D-alanine--D-alanine ligase -
LE166_RS02185 (LE166_02185) 444042..445400 + 1359 WP_002492089.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(19-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-90)


Sequences

Toxin        


Download         Length: 71 a.a.        Molecular weight: 8087.02 Da        Isoelectric Point: 3.9947

>T218637 WP_002492077.1 NZ_CP084437:440554-440766 [Staphylococcus lugdunensis]
VEKRLIYITNVTNEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETSTNLNALEKEIYDAGYHAIDDL

Download         Length: 213 bp

>T218637 NZ_CP116067:c4810729-4810511 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11177.85 Da        Isoelectric Point: 6.6367

>AT218637 WP_002492098.1 NZ_CP084437:440248-440541 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Download         Length: 294 bp

>AT218637 NZ_CP116067:c4811129-4810755 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGATCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V532

Antitoxin

Source ID Structure
AlphaFold DB A0A853V130

References