Detailed information of TA system TA218636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 724225..724743
• Replicon: chromosome
• Accession: NZ_CP084434
• Organism: Staphylococcus lugdunensis strain RMLUG2

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: LE165_RS03570
• Protein ID: WP_002478973.1
• Coordinates: 724225..724437 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A853V130
• Locus tag: LE165_RS03575
• Protein ID: WP_002492098.1
• Coordinates: 724450..724743 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE165_RS03550 (LE165_03560)	719591..720949	-	1359	WP_037544984.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
LE165_RS03555 (LE165_03565)	720967..722037	-	1071	WP_084951667.1	D-alanine--D-alanine ligase	-
LE165_RS03560 (LE165_03570)	722393..723583	+	1191	WP_002461205.1	FtsW/RodA/SpoVE family cell cycle protein	-
LE165_RS03565 (LE165_03575)	723997..724134	-	138	WP_002461206.1	Lmo0850 family protein	-
LE165_RS03570 (LE165_03580)	724225..724437	-	213	WP_002478973.1	heavy metal-associated domain-containing protein	Toxin
LE165_RS03575 (LE165_03585)	724450..724743	-	294	WP_002492098.1	copper-sensing transcriptional repressor CsoR	Antitoxin
LE165_RS03580 (LE165_03590)	724909..726393	+	1485	WP_002478972.1	cardiolipin synthase	-
LE165_RS03585 (LE165_03595)	726515..727156	+	642	WP_084951665.1	HD domain-containing protein	-
LE165_RS03590 (LE165_03600)	727540..728415	-	876	WP_002461212.1	membrane protein insertase YidC	-
LE165_RS03595 (LE165_03605)	728505..729143	-	639	WP_002461213.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (19-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-90)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8070.03 Da
• Isoelectric Point: 3.9947

>T218636 WP_002478973.1 NZ_CP084434:c724437-724225 [Staphylococcus lugdunensis]
VEKRLIYITNVTSEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETPTNLNALEKEIYDAGYHAIDDL

Nucleotide

• Length: 213 bp

>T218636 NZ_CP116067:4165499-4165873 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTTGG
CCGTCTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGTGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11177.85 Da
• Isoelectric Point: 6.6367

>AT218636 WP_002492098.1 NZ_CP084434:c724743-724450 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Nucleotide

• Length: 294 bp

>AT218636 NZ_CP116067:4165041-4165409 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACTCATCCCGATGACAACAACAGCCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAGCTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTTACCTGCAAAGCCGATACCCTCGGCAGTCG
AGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A853V130