Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 712825..713342 Replicon chromosome
Accession NZ_CP084434
Organism Staphylococcus lugdunensis strain RMLUG2

Toxin (Protein)


Gene name mazF Uniprot ID A0A853V3R6
Locus tag LE165_RS03510 Protein ID WP_002461192.1
Coordinates 712825..713175 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A853V233
Locus tag LE165_RS03515 Protein ID WP_002461193.1
Coordinates 713172..713342 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE165_RS03490 (LE165_03500) 709750..710520 - 771 WP_252920622.1 RNA polymerase sigma factor SigB -
LE165_RS03495 (LE165_03505) 710495..710971 - 477 WP_267263128.1 anti-sigma B factor RsbW -
LE165_RS03500 (LE165_03510) 710973..711299 - 327 WP_002461190.1 anti-sigma factor antagonist -
LE165_RS03505 (LE165_03515) 711390..712391 - 1002 WP_267263129.1 PP2C family protein-serine/threonine phosphatase -
LE165_RS03510 (LE165_03520) 712825..713175 - 351 WP_002461192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LE165_RS03515 (LE165_03525) 713172..713342 - 171 WP_002461193.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LE165_RS03520 (LE165_03530) 713429..714577 - 1149 WP_062077878.1 alanine racemase -
LE165_RS03525 (LE165_03535) 714625..715017 - 393 WP_002461196.1 holo-ACP synthase -
LE165_RS03530 (LE165_03540) 715029..715541 - 513 WP_002478976.1 PH domain-containing protein -
LE165_RS03535 (LE165_03545) 715528..717042 - 1515 WP_084951668.1 PH domain-containing protein -
LE165_RS03540 (LE165_03550) 717035..717517 - 483 WP_070481305.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13079.20 Da        Isoelectric Point: 9.9784

>T218635 WP_002461192.1 NZ_CP084434:c713175-712825 [Staphylococcus lugdunensis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIGKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSEDKMKEVDHALDISLGLHNYH

Download         Length: 351 bp

>T218635 NZ_CP116067:4057744-4058094 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTACTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6327.92 Da        Isoelectric Point: 4.0115

>AT218635 WP_002461193.1 NZ_CP084434:c713342-713172 [Staphylococcus lugdunensis]
MLTFNQNRSHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNHQSE

Download         Length: 171 bp

>AT218635 NZ_CP116067:4057499-4057750 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V3R6

Antitoxin

Source ID Structure
AlphaFold DB A0A853V233

References