Detailed information of TA system TA218633

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3258643..3259306
• Replicon: chromosome
• Accession: NZ_CP084433
• Organism: Edwardsiella piscicida strain S07-534

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: DCF40_RS15140
• Protein ID: WP_045474345.1
• Coordinates: 3258643..3259011 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: D0ZDY6
• Locus tag: DCF40_RS15145
• Protein ID: WP_012849755.1
• Coordinates: 3259040..3259306 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF40_RS15120 (DCF40_15120)	3254573..3256306	-	1734	WP_225857321.1	single-stranded-DNA-specific exonuclease RecJ	-
DCF40_RS15125 (DCF40_15125)	3256311..3257027	-	717	WP_015462274.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
DCF40_RS15130 (DCF40_15130)	3257055..3257955	-	901	Protein_2915	site-specific tyrosine recombinase XerD	-
DCF40_RS15135 (DCF40_15135)	3258106..3258618	+	513	WP_012849753.1	flavodoxin FldB	-
DCF40_RS15140 (DCF40_15140)	3258643..3259011	-	369	WP_045474345.1	protein YgfX	Toxin
DCF40_RS15145 (DCF40_15145)	3259040..3259306	-	267	WP_012849755.1	FAD assembly factor SdhE	Antitoxin
DCF40_RS15150 (DCF40_15150)	3259654..3260652	+	999	WP_226088122.1	tRNA-modifying protein YgfZ	-
DCF40_RS15155 (DCF40_15155)	3260695..3261519	-	825	WP_069579303.1	shikimate 5-dehydrogenase	-
DCF40_RS15160 (DCF40_15160)	3261678..3262550	-	873	WP_012849758.1	nucleoside-specific channel-forming protein Tsx	-
DCF40_RS15165 (DCF40_15165)	3263159..3263809	+	651	WP_069579679.1	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-115)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14248.71 Da
• Isoelectric Point: 11.3493

>T218633 WP_045474345.1 NZ_CP084433:c3259011-3258643 [Edwardsiella piscicida]
MFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRRIARNRGELQLLAPQVWHWQLREWRLARRPWVSDLGAL
LILQSTHGAPCRRRLWLAADSMSREEWGQLRRTLLDAGDDRA

Nucleotide

• Length: 369 bp

>T218633 NZ_CP116067:3602547-3602858 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTAGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAGGCAATGCTCCAGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10318.90 Da
• Isoelectric Point: 5.6951

>AT218633 WP_012849755.1 NZ_CP084433:c3259306-3259040 [Edwardsiella piscicida]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLACDDPELFKWLMNQGKPDDADLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT218633 NZ_CP116067:3602859-3603149 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A034T2Q8