Detailed information of TA system TA218612

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3100444..3101126
• Replicon: chromosome
• Accession: NZ_CP084432
• Organism: Edwardsiella tarda strain AL98-87

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: DCF75_RS14345
• Protein ID: WP_116605309.1
• Coordinates: 3100444..3100785 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DCF75_RS14350
• Protein ID: WP_116605310.1
• Coordinates: 3100806..3101126 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF75_RS14325 (DCF75_14325)	3095663..3097411	+	1749	WP_035597148.1	DNA primase	-
DCF75_RS14330 (DCF75_14330)	3097647..3099488	+	1842	WP_005281642.1	RNA polymerase sigma factor RpoD	-
DCF75_RS14335 (DCF75_14335)	3099577..3100071	-	495	WP_068871231.1	G/U mismatch-specific DNA glycosylase	-
DCF75_RS14345 (DCF75_14345)	3100444..3100785	-	342	WP_116605309.1	TA system toxin CbtA family protein	Toxin
DCF75_RS14350 (DCF75_14350)	3100806..3101126	-	321	WP_116605310.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DCF75_RS14355 (DCF75_14355)	3101145..3101366	-	222	WP_116605311.1	DUF987 domain-containing protein	-
DCF75_RS14360 (DCF75_14360)	3101375..3101857	-	483	WP_116605312.1	RadC family protein	-
DCF75_RS14365 (DCF75_14365)	3101866..3102324	-	459	WP_116605313.1	antirestriction protein	-
DCF75_RS14370 (DCF75_14370)	3102410..3102646	-	237	WP_116605314.1	DUF905 domain-containing protein	-
DCF75_RS14375 (DCF75_14375)	3102724..3103134	-	411	WP_116605315.1	hypothetical protein	-
DCF75_RS14380 (DCF75_14380)	3103201..3103638	-	438	WP_116605316.1	hypothetical protein	-
DCF75_RS14385 (DCF75_14385)	3103679..3104215	-	537	WP_116605317.1	DUF4339 domain-containing protein	-
DCF75_RS14390 (DCF75_14390)	3104241..3104951	-	711	WP_116605318.1	DeoR family transcriptional regulator	-
DCF75_RS14395 (DCF75_14395)	3105159..3105971	-	813	WP_116605319.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-105)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12823.74 Da
• Isoelectric Point: 9.0057

>T218612 WP_116605309.1 NZ_CP084432:c3100785-3100444 [Edwardsiella tarda]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVDKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLRRSRNNAVS

Nucleotide

• Length: 342 bp

>T218612 NZ_CP116046:c3986722-3986444 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCATCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12173.70 Da
• Isoelectric Point: 6.8318

>AT218612 WP_116605310.1 NZ_CP084432:c3101126-3100806 [Edwardsiella tarda]
MRSHVTWGLQRDITPHLGTRLVQEGNRLHYLADRASITGKFSDAEFRKLDETFPHFISQMESMLTTDELNPRHAHCVTLY
HNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 321 bp

>AT218612 NZ_CP116046:c3986985-3986725 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA