Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2571577..2572169 Replicon chromosome
Accession NZ_CP084432
Organism Edwardsiella tarda strain AL98-87

Toxin (Protein)


Gene name Hha Uniprot ID D4F316
Locus tag DCF75_RS12000 Protein ID WP_005283517.1
Coordinates 2571966..2572169 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID D4F317
Locus tag DCF75_RS11995 Protein ID WP_005283520.1
Coordinates 2571577..2571945 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DCF75_RS11985 (DCF75_11985) 2566819..2568003 + 1185 WP_005296656.1 efflux RND transporter periplasmic adaptor subunit -
DCF75_RS11990 (DCF75_11990) 2568048..2571203 + 3156 WP_005283526.1 efflux RND transporter permease subunit -
DCF75_RS11995 (DCF75_11995) 2571577..2571945 + 369 WP_005283520.1 Hha toxicity modulator TomB Antitoxin
DCF75_RS12000 (DCF75_12000) 2571966..2572169 + 204 WP_005283517.1 HHA domain-containing protein Toxin
DCF75_RS12010 (DCF75_12010) 2573068..2573385 + 318 WP_035600211.1 MGMT family protein -
DCF75_RS12015 (DCF75_12015) 2573466..2574332 + 867 WP_068869964.1 acyl-CoA thioesterase II -
DCF75_RS12020 (DCF75_12020) 2574357..2575615 - 1259 Protein_2318 ammonium transporter AmtB -
DCF75_RS12025 (DCF75_12025) 2575660..2575998 - 339 WP_226085969.1 P-II family nitrogen regulator -
DCF75_RS12030 (DCF75_12030) 2576278..2576739 - 462 WP_005296844.1 Lrp/AsnC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8088.36 Da        Isoelectric Point: 5.8288

>T218611 WP_005283517.1 NZ_CP084432:2571966-2572169 [Edwardsiella tarda]
MTKIDYLMKLRKCTSLETLERVIEKNKYELSNDELEVFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Download         Length: 204 bp

>T218611 NZ_CP116046:3681267-3681554 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14260.05 Da        Isoelectric Point: 6.0582

>AT218611 WP_005283520.1 NZ_CP084432:2571577-2571945 [Edwardsiella tarda]
MDEYTSYRHDIAELKYLCDSLYRQGMDVLEESHHGWVSDPTAMVNLQLNELIEHIANVALSFKIKYPQHSSLCEIVDDYL
DETYALFSAYSVSEQALRHWLRSKRRVAYSLAHEKRNAALHV

Download         Length: 369 bp

>AT218611 NZ_CP116046:3681541-3681843 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4F316

Antitoxin

Source ID Structure
AlphaFold DB D4F317

References