Detailed information of TA system TA021861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1045156..1045775
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BCG_RS04955
• Protein ID: WP_003404726.1
• Coordinates: 1045341..1045775 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: BCG_RS04950
• Protein ID: WP_003404724.1
• Coordinates: 1045156..1045335 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS04940	1040629..1042209	+	1581	WP_011799139.1	serine hydrolase	-
BCG_RS04945	1042206..1044599	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
BCG_RS22360	1044872..1045030	+	159	WP_003404720.1	hypothetical protein	-
BCG_RS04950	1045156..1045335	+	180	WP_003404724.1	antitoxin	Antitoxin
BCG_RS04955	1045341..1045775	+	435	WP_003404726.1	SRPBCC family protein	Toxin
BCG_RS04960	1045873..1046646	+	774	WP_003404735.1	VOC family protein	-
BCG_RS04965	1046711..1047160	+	450	WP_003404738.1	hypothetical protein	-
BCG_RS04970	1047295..1047525	-	231	WP_003898642.1	hypothetical protein	-
BCG_RS04975	1047692..1049200	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
BCG_RS04980	1049202..1050440	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T21861 WP_003404726.1 NC_008769:1045341-1045775 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T21861 NC_008769:1045341-1045775 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT21861 WP_003404724.1 NC_008769:1045156-1045335 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT21861 NC_008769:1045156-1045335 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5