Detailed information of TA system TA218599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8..13652
• Replicon: plasmid pEP-S07-348-1
• Accession: NZ_CP084431
• Organism: Edwardsiella piscicida strain S07-348

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DCF39_RS17745
• Protein ID: WP_009873354.1
• Coordinates: 8..334 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DCF39_RS17740
• Protein ID: WP_043149934.1
• Coordinates: 13652..11 (+)
• Length: -4546.6666666667 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF39_RS17745 (DCF39_17760)	8..334	+	327	WP_009873354.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DCF39_RS17750 (DCF39_17765)	331..657	+	327	WP_009873353.1	hypothetical protein	-
DCF39_RS17755 (DCF39_17770)	686..1564	+	879	WP_009873352.1	helicase RepA family protein	-
DCF39_RS17760 (DCF39_17775)	1545..2537	+	993	WP_009873351.1	replication protein C, IncQ-type	-
DCF39_RS17765 (DCF39_17780)	2590..3969	-	1380	WP_009873366.1	RNA-guided endonuclease TnpB family protein	-
DCF39_RS17770 (DCF39_17785)	4046..4501	+	456	WP_009873365.1	IS200/IS605 family transposase	-
DCF39_RS17775 (DCF39_17790)	4524..5159	-	636	WP_047706566.1	tetracycline resistance transcriptional repressor TetR	-
DCF39_RS17780 (DCF39_17795)	5265..6455	+	1191	WP_047706567.1	tetracycline efflux MFS transporter Tet(A)	-
DCF39_RS17785 (DCF39_17800)	6359..6697	+	339	WP_071845138.1	hypothetical protein	-
DCF39_RS17790 (DCF39_17805)	6905..7210	+	306	WP_009873361.1	tyrosine-type recombinase/integrase	-
DCF39_RS17795 (DCF39_17810)	7863..8615	-	753	WP_009873360.1	hypothetical protein	-
DCF39_RS17800 (DCF39_17815)	8612..9250	-	639	WP_009873359.1	hypothetical protein	-
DCF39_RS17805 (DCF39_17820)	9243..9926	-	684	WP_009873358.1	protein mobD	-
DCF39_RS17810 (DCF39_17825)	9939..10295	-	357	WP_009873357.1	hypothetical protein	-
DCF39_RS17815 (DCF39_17830)	10608..10922	+	315	WP_223526267.1	ribbon-helix-helix protein, CopG family	-
DCF39_RS17820 (DCF39_17835)	10912..13563	+	2652	WP_227015412.1	DNA-primase RepB domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A)	-	1..13865	13865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11487.49 Da
• Isoelectric Point: 9.2954

>T218599 WP_009873354.1 NZ_CP084431:8-334 [Edwardsiella piscicida]
MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLNAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSK
VGPAFGRIDADALLEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T218599 NZ_CP116046:547800-547976 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: -4546.6666666667 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT218599 WP_043149934.1 NZ_CP084431:13652-11 [Edwardsiella piscicida]

Nucleotide

• Length: -13640 bp

>AT218599 NZ_CP116046:548022-548438 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA