Detailed information of TA system TA218579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3269331..3271051
• Replicon: chromosome
• Accession: NZ_CP084429
• Organism: Edwardsiella piscicida strain S07-346

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A411H3H5
• Locus tag: DCF38_RS14985
• Protein ID: WP_015683486.1
• Coordinates: 3270395..3271051 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: DCF38_RS14980
• Protein ID: WP_226081859.1
• Coordinates: 3269331..3270359 (-)
• Length: 343 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF38_RS14970 (DCF38_14970)	3265356..3266072	-	717	WP_116603502.1	SprT family zinc-dependent metalloprotease	-
DCF38_RS14975 (DCF38_14975)	3266072..3269167	-	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
DCF38_RS14980 (DCF38_14980)	3269331..3270359	-	1029	WP_226081859.1	macro domain-containing protein	Antitoxin
DCF38_RS14985 (DCF38_14985)	3270395..3271051	-	657	WP_015683486.1	DUF4433 domain-containing protein	Toxin
DCF38_RS14990 (DCF38_14990)	3271064..3272413	-	1350	WP_080545142.1	restriction endonuclease subunit S	-
DCF38_RS14995 (DCF38_14995)	3272425..3274863	-	2439	WP_015683488.1	type I restriction-modification system subunit M	-
DCF38_RS15000 (DCF38_15000)	3274902..3275141	-	240	WP_012849883.1	hypothetical protein	-
DCF38_RS15005 (DCF38_15005)	3275493..3275849	-	357	Protein_2895	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3248874..3289248	40374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (5-116)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25046.43 Da
• Isoelectric Point: 6.9045

>T218579 WP_015683486.1 NZ_CP084429:c3271051-3270395 [Edwardsiella piscicida]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSQVQAVNWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSSLRSVAAQNAPFVFTDSHAYYNWANYYTRLEDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T218579 NZ_CP116044:c2162272-2162165 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 343 a.a.
• Molecular weight: 39249.39 Da
• Isoelectric Point: 7.1506

>AT218579 WP_226081859.1 NZ_CP084429:c3270359-3269331 [Edwardsiella piscicida]
VEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTKQHWRADSRMAWIE
DGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSSGVKKLTPARAMIA
ELVRRYWVLGMECSLLEIQKLAWLLQRAIELHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDV
IWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEGLHQWPAGEPWASR
KIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1029 bp

>AT218579 NZ_CP116044:2162325-2162386 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A411H3H5