Detailed information of TA system TA218575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3272520..3274240
• Replicon: chromosome
• Accession: NZ_CP084428
• Organism: Edwardsiella piscicida strain S07-275

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: DCF36_RS15040
• Protein ID: WP_116541315.1
• Coordinates: 3273584..3274240 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: DCF36_RS15035
• Protein ID: WP_226079113.1
• Coordinates: 3272520..3273548 (-)
• Length: 343 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCF36_RS15025 (DCF36_15025)	3268690..3269406	-	717	WP_116541312.1	SprT family zinc-dependent metalloprotease	-
DCF36_RS15030 (DCF36_15030)	3269406..3272501	-	3096	WP_116541313.1	HsdR family type I site-specific deoxyribonuclease	-
DCF36_RS15035 (DCF36_15035)	3272520..3273548	-	1029	WP_226079113.1	macro domain-containing protein	Antitoxin
DCF36_RS15040 (DCF36_15040)	3273584..3274240	-	657	WP_116541315.1	DUF4433 domain-containing protein	Toxin
DCF36_RS15045 (DCF36_15045)	3274243..3274818	-	576	WP_012849886.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
DCF36_RS15050 (DCF36_15050)	3274823..3276172	-	1350	WP_116541322.1	restriction endonuclease subunit S	-
DCF36_RS15055 (DCF36_15055)	3276172..3278610	-	2439	WP_116541316.1	type I restriction-modification system subunit M	-
DCF36_RS15060 (DCF36_15060)	3278649..3278888	-	240	WP_012849883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3272520..3292374	19854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (5-116)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25136.49 Da
• Isoelectric Point: 6.6894

>T218575 WP_116541315.1 NZ_CP084428:c3274240-3273584 [Edwardsiella piscicida]
VAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGKSPVQAANWVNIGNPELIDKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAQNVPFVFTDSHAYYNWANYYTRLDELNQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLDGMVCYNNDVKVQLEDWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T218575 NZ_CP116044:c1066910-1066632 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 343 a.a.
• Molecular weight: 39282.40 Da
• Isoelectric Point: 7.1506

>AT218575 WP_226079113.1 NZ_CP084428:c3273548-3272520 [Edwardsiella piscicida]
VEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTKQHWRADSRMAWIE
DGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSSGVKKLTPARAMIA
ELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDV
IWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEGLHQWPAGEPWASR
KMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1029 bp

>AT218575 NZ_CP116044:c1067173-1066913 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA