Detailed information of TA system TA218549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 6154..6689
• Replicon: plasmid p1
• Accession: NZ_CP084416
• Organism: Klebsiella michiganensis strain YL6-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: LDP80_RS29135
• Protein ID: WP_032693963.1
• Coordinates: 6154..6441 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LDP80_RS29140
• Protein ID: WP_032693964.1
• Coordinates: 6438..6689 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDP80_RS29105 (LDP80_29145)	1744..2376	+	633	WP_080884176.1	ParA family protein	-
LDP80_RS29110 (LDP80_29150)	2376..2750	+	375	WP_088903436.1	hypothetical protein	-
LDP80_RS29115 (LDP80_29155)	2990..3964	+	975	WP_080884175.1	plasmid segregation protein ParM	-
LDP80_RS29120 (LDP80_29160)	3968..4360	+	393	WP_080884174.1	plasmid partitioning/stability family protein	-
LDP80_RS29125 (LDP80_29165)	4636..5136	-	501	WP_080884173.1	PBECR2 nuclease fold domain-containing protein	-
LDP80_RS29130 (LDP80_29170)	5883..6071	-	189	WP_080884172.1	hypothetical protein	-
LDP80_RS29135 (LDP80_29175)	6154..6441	-	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDP80_RS29140 (LDP80_29180)	6438..6689	-	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDP80_RS29145 (LDP80_29185)	6856..8130	-	1275	WP_080884171.1	Y-family DNA polymerase	-
LDP80_RS29150 (LDP80_29190)	8130..8555	-	426	WP_032693966.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LDP80_RS29155 (LDP80_29195)	8765..8995	+	231	WP_032693967.1	hypothetical protein	-
LDP80_RS29160 (LDP80_29200)	9430..9663	+	234	Protein_12	hypothetical protein	-
LDP80_RS29165 (LDP80_29205)	9660..9995	+	336	WP_080884169.1	hypothetical protein	-
LDP80_RS29170 (LDP80_29210)	10087..10259	+	173	Protein_14	hypothetical protein	-
LDP80_RS29175 (LDP80_29215)	10379..11080	+	702	WP_080884168.1	DNA methylase	-
LDP80_RS29180 (LDP80_29220)	11080..11301	+	222	WP_053390281.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..101530	101530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T218549 WP_032693963.1 NZ_CP084416:c6441-6154 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T218549 NZ_CP116036:c39431-39153 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT218549 WP_032693964.1 NZ_CP084416:c6689-6438 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT218549 NZ_CP116036:c39700-39431 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2