Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5392804..5393608 Replicon chromosome
Accession NZ_CP084415
Organism Klebsiella michiganensis strain YL6-1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A486SQP3
Locus tag LDP80_RS25725 Protein ID WP_040217122.1
Coordinates 5392804..5393193 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7H4M120
Locus tag LDP80_RS25730 Protein ID WP_014837280.1
Coordinates 5393249..5393608 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDP80_RS25695 (LDP80_25720) 5387846..5388640 + 795 WP_042933970.1 DNA/RNA non-specific endonuclease -
LDP80_RS25700 (LDP80_25725) 5388930..5389253 + 324 WP_014837285.1 endoribonuclease SymE -
LDP80_RS25705 (LDP80_25730) 5389412..5389846 + 435 WP_004098173.1 VOC family protein -
LDP80_RS25710 (LDP80_25735) 5390012..5390755 + 744 WP_014837284.1 hypothetical protein -
LDP80_RS25715 (LDP80_25740) 5390933..5391730 - 798 WP_014837283.1 helix-turn-helix transcriptional regulator -
LDP80_RS25720 (LDP80_25745) 5391872..5392717 - 846 WP_014837282.1 DUF4942 domain-containing protein -
LDP80_RS25725 (LDP80_25750) 5392804..5393193 - 390 WP_040217122.1 TA system toxin CbtA family protein Toxin
LDP80_RS25730 (LDP80_25755) 5393249..5393608 - 360 WP_014837280.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LDP80_RS25735 (LDP80_25760) 5393633..5393854 - 222 WP_014837279.1 DUF987 domain-containing protein -
LDP80_RS25740 (LDP80_25765) 5393868..5394347 - 480 WP_014837278.1 DNA repair protein RadC -
LDP80_RS25745 (LDP80_25770) 5394359..5394802 - 444 WP_014837277.1 antirestriction protein -
LDP80_RS25750 (LDP80_25775) 5394882..5395112 - 231 WP_014837276.1 DUF905 domain-containing protein -
LDP80_RS25755 (LDP80_25780) 5395136..5395960 - 825 WP_040217125.1 DUF932 domain-containing protein -
LDP80_RS25760 (LDP80_25785) 5396500..5397132 + 633 WP_071886307.1 inovirus Gp2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5389412..5428560 39148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(3-111)

Antitoxin

Antitoxin CbeA

(16-114)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14477.57 Da        Isoelectric Point: 8.9843

>T218548 WP_040217122.1 NZ_CP084415:c5393193-5392804 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T218548 NZ_CP116036:c2588-2436 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13416.24 Da        Isoelectric Point: 7.0268

>AT218548 WP_014837280.1 NZ_CP084415:c5393608-5393249 [Klebsiella michiganensis]
MSKKTTIATHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Download         Length: 360 bp

>AT218548 NZ_CP116036:2638-2700 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486SQP3

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4M120

References