Detailed information of TA system TA218547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5336600..5337176
• Replicon: chromosome
• Accession: NZ_CP084415
• Organism: Klebsiella michiganensis strain YL6-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: LDP80_RS25465
• Protein ID: WP_042933975.1
• Coordinates: 5336889..5337176 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LDP80_RS25460
• Protein ID: WP_014837314.1
• Coordinates: 5336600..5336902 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDP80_RS25440 (LDP80_25465)	5332102..5333322	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
LDP80_RS25445 (LDP80_25470)	5333440..5333775	+	336	WP_014837316.1	endoribonuclease SymE	-
LDP80_RS25450 (LDP80_25475)	5334228..5335139	+	912	WP_014837315.1	acetamidase/formamidase family protein	-
LDP80_RS25455 (LDP80_25480)	5335136..5336479	+	1344	WP_004128506.1	APC family permease	-
LDP80_RS25460 (LDP80_25485)	5336600..5336902	-	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
LDP80_RS25465 (LDP80_25490)	5336889..5337176	-	288	WP_042933975.1	BrnT family toxin	Toxin
LDP80_RS25470 (LDP80_25495)	5337435..5337878	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
LDP80_RS25475 (LDP80_25500)	5337872..5338780	-	909	WP_014837311.1	LysR family transcriptional regulator	-
LDP80_RS25480 (LDP80_25505)	5338868..5339650	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
LDP80_RS25485 (LDP80_25510)	5339798..5340382	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
LDP80_RS25490 (LDP80_25515)	5340528..5341328	+	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
LDP80_RS25495 (LDP80_25520)	5341325..5341843	+	519	WP_014837307.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	fosA	-	5332102..5337878	5776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T218547 WP_042933975.1 NZ_CP084415:c5337176-5336889 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T218547 NZ_CP116035:c4839132-4838821 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT218547 WP_014837314.1 NZ_CP084415:c5336902-5336600 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT218547 NZ_CP116035:c4838820-4838530 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2