Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4641407..4642026 Replicon chromosome
Accession NZ_CP084415
Organism Klebsiella michiganensis strain YL6-1

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LDP80_RS22235 Protein ID WP_004099646.1
Coordinates 4641808..4642026 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag LDP80_RS22230 Protein ID WP_004129911.1
Coordinates 4641407..4641781 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDP80_RS22220 (LDP80_22245) 4636565..4637758 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LDP80_RS22225 (LDP80_22250) 4637781..4640927 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LDP80_RS22230 (LDP80_22255) 4641407..4641781 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
LDP80_RS22235 (LDP80_22260) 4641808..4642026 + 219 WP_004099646.1 HHA domain-containing protein Toxin
LDP80_RS22240 (LDP80_22265) 4642189..4642755 + 567 WP_014837607.1 maltose O-acetyltransferase -
LDP80_RS22245 (LDP80_22270) 4642727..4642861 - 135 WP_223226764.1 hypothetical protein -
LDP80_RS22250 (LDP80_22275) 4642882..4643352 + 471 WP_014228205.1 YlaC family protein -
LDP80_RS22255 (LDP80_22280) 4643327..4644781 - 1455 WP_014837606.1 PLP-dependent aminotransferase family protein -
LDP80_RS22260 (LDP80_22285) 4644883..4645581 + 699 WP_014228203.1 GNAT family protein -
LDP80_RS22265 (LDP80_22290) 4645578..4645718 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LDP80_RS22270 (LDP80_22295) 4645718..4645981 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T218545 WP_004099646.1 NZ_CP084415:4641808-4642026 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T218545 NZ_CP116035:4495323-4495697 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT218545 WP_004129911.1 NZ_CP084415:4641407-4641781 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT218545 NZ_CP116035:4494865-4495233 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References