Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 747296..747945 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag BCG_RS03425 Protein ID WP_003403236.1
Coordinates 747550..747945 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag BCG_RS03420 Protein ID WP_003403235.1
Coordinates 747296..747550 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS22205 742421..742504 + 84 Protein_673 galactose-1-phosphate uridylyltransferase -
BCG_RS03395 742523..743605 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
BCG_RS03400 743602..744693 + 1092 WP_003403225.1 galactokinase -
BCG_RS03410 745088..746245 + 1158 WP_003903091.1 hypothetical protein -
BCG_RS03415 746256..747203 + 948 WP_003403232.1 DUF732 domain-containing protein -
BCG_RS03420 747296..747550 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
BCG_RS03425 747550..747945 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
BCG_RS03430 748039..748779 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
BCG_RS03435 748911..749171 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BCG_RS03440 749168..749575 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
BCG_RS03445 749647..750798 - 1152 WP_003403248.1 FIST C-terminal domain-containing protein -
BCG_RS03450 750891..752618 - 1728 WP_010950423.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T21854 WP_003403236.1 NC_008769:747550-747945 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T21854 NC_008769:747550-747945 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT21854 WP_003403235.1 NC_008769:747296-747550 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT21854 NC_008769:747296-747550 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References