Detailed information of TA system TA218535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 56966..57513
• Replicon: chromosome
• Accession: NZ_CP084415
• Organism: Klebsiella michiganensis strain YL6-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LDP80_RS00290
• Protein ID: WP_014839830.1
• Coordinates: 56966..57274 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3H0P7
• Locus tag: LDP80_RS00295
• Protein ID: WP_014227298.1
• Coordinates: 57277..57513 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDP80_RS00260 (LDP80_00260)	52613..52924	-	312	WP_014839834.1	PTS sugar transporter subunit IIB	-
LDP80_RS00265 (LDP80_00265)	53219..54160	+	942	WP_014227303.1	LacI family DNA-binding transcriptional regulator	-
LDP80_RS00270 (LDP80_00270)	54184..54477	-	294	WP_014227302.1	YicS family protein	-
LDP80_RS00275 (LDP80_00275)	54687..55652	+	966	WP_014839832.1	Rpn family recombination-promoting nuclease/putative transposase	-
LDP80_RS00280 (LDP80_00280)	55727..55909	+	183	WP_014839831.1	hypothetical protein	-
LDP80_RS00285 (LDP80_00285)	55913..56815	-	903	WP_014227300.1	EamA family transporter	-
LDP80_RS00290 (LDP80_00290)	56966..57274	-	309	WP_014839830.1	CcdB family protein	Toxin
LDP80_RS00295 (LDP80_00295)	57277..57513	-	237	WP_014227298.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LDP80_RS00300 (LDP80_00300)	57619..59052	-	1434	WP_014839829.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
LDP80_RS00305 (LDP80_00305)	59077..59979	-	903	WP_009654080.1	N-acetylmuramic acid 6-phosphate etherase	-
LDP80_RS00310 (LDP80_00310)	60140..61306	-	1167	WP_014839828.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11724.63 Da
• Isoelectric Point: 8.8012

>T218535 WP_014839830.1 NZ_CP084415:c57274-56966 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGKE
VCNLNHQREVVKASMDFLFDGI

Nucleotide

• Length: 309 bp

>T218535 NZ_CP116035:2148617-2148719 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8783.92 Da
• Isoelectric Point: 5.7928

>AT218535 WP_014227298.1 NZ_CP084415:c57513-57277 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT218535 NZ_CP116035:c2148757-2148489 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A0H3H0P7