Detailed information of TA system TA218532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4856338..4856933
• Replicon: chromosome
• Accession: NZ_CP084413
• Organism: Pseudomonas sp. HS-18

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: LDJ84_RS22605
• Protein ID: WP_226045432.1
• Coordinates: 4856338..4856679 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: LDJ84_RS22610
• Protein ID: WP_226045433.1
• Coordinates: 4856685..4856933 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDJ84_RS22570 (LDJ84_22570)	4852202..4852966	-	765	WP_065085981.1	RES family NAD+ phosphorylase	-
LDJ84_RS22575 (LDJ84_22575)	4852967..4853335	-	369	WP_024763405.1	DUF2384 domain-containing protein	-
LDJ84_RS22580 (LDJ84_22580)	4853897..4854097	+	201	WP_226045429.1	RcnB family protein	-
LDJ84_RS22585 (LDJ84_22585)	4854104..4854547	-	444	WP_226045430.1	hypothetical protein	-
LDJ84_RS22590 (LDJ84_22590)	4854672..4855154	+	483	WP_226045431.1	GreA/GreB family elongation factor	-
LDJ84_RS22595 (LDJ84_22595)	4855288..4855635	+	348	WP_017522075.1	carboxymuconolactone decarboxylase family protein	-
LDJ84_RS22600 (LDJ84_22600)	4855735..4856103	+	369	WP_037015850.1	hypothetical protein	-
LDJ84_RS22605 (LDJ84_22605)	4856338..4856679	-	342	WP_226045432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDJ84_RS22610 (LDJ84_22610)	4856685..4856933	-	249	WP_226045433.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDJ84_RS22615 (LDJ84_22615)	4857034..4858308	-	1275	WP_226045434.1	hypothetical protein	-
LDJ84_RS22620 (LDJ84_22620)	4858550..4859839	-	1290	WP_226045435.1	zonular occludens toxin domain-containing protein	-
LDJ84_RS22625 (LDJ84_22625)	4859843..4860199	-	357	WP_226045436.1	DUF2523 family protein	-
LDJ84_RS22630 (LDJ84_22630)	4860203..4861078	-	876	WP_226045437.1	permease	-
LDJ84_RS22635 (LDJ84_22635)	4861681..4861920	-	240	WP_226045438.1	major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13004.69 Da
• Isoelectric Point: 4.5106

>T218532 WP_226045432.1 NZ_CP084413:c4856679-4856338 [Pseudomonas sp. HS-18]
MTVREIRFTETAVFSIQDQEEHLADYHPPELAAAKIDGLIDEILTRLQDAPVGYPVSRQASDLGVTRYRELNHDGYRVFY
EVYEHENVIAVELVLRQKQDVEAALIRYCLVGI

Nucleotide

• Length: 342 bp

>T218532 NZ_CP116035:1101045-1101380 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9326.74 Da
• Isoelectric Point: 9.5977

>AT218532 WP_226045433.1 NZ_CP084413:c4856933-4856685 [Pseudomonas sp. HS-18]
MRVETISYLKRHAADLDLDEPMVVTQNGVPTLVVESYAERKRRDQAIALVKLLAMGSREYAEGKHCSADELKARLAKRRT
QQ

Nucleotide

• Length: 249 bp

>AT218532 NZ_CP116035:1100797-1101045 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA