Detailed information of TA system TA218530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1394142..1394731
• Replicon: chromosome
• Accession: NZ_CP084413
• Organism: Pseudomonas sp. HS-18

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LDJ84_RS06555
• Protein ID: WP_226046095.1
• Coordinates: 1394423..1394731 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1B8TB76
• Locus tag: LDJ84_RS06550
• Protein ID: WP_037011255.1
• Coordinates: 1394142..1394438 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDJ84_RS06525 (LDJ84_06525)	1389476..1390114	-	639	WP_226046091.1	hypothetical protein	-
LDJ84_RS06530 (LDJ84_06530)	1390251..1391561	-	1311	WP_226046092.1	HAMP domain-containing sensor histidine kinase	-
LDJ84_RS06535 (LDJ84_06535)	1391558..1392298	-	741	WP_024767564.1	response regulator	-
LDJ84_RS06540 (LDJ84_06540)	1392524..1392796	+	273	WP_226046093.1	DUF2790 domain-containing protein	-
LDJ84_RS06545 (LDJ84_06545)	1392890..1394110	+	1221	WP_226046094.1	cytochrome c biogenesis protein DipZ	-
LDJ84_RS06550 (LDJ84_06550)	1394142..1394438	-	297	WP_037011255.1	putative addiction module antidote protein	Antitoxin
LDJ84_RS06555 (LDJ84_06555)	1394423..1394731	-	309	WP_226046095.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDJ84_RS06560 (LDJ84_06560)	1394848..1396695	-	1848	WP_226046096.1	diguanylate cyclase	-
LDJ84_RS06565 (LDJ84_06565)	1396977..1397111	-	135	Protein_1298	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1387882..1440637	52755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (7-93)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11619.39 Da
• Isoelectric Point: 10.3398

>T218530 WP_226046095.1 NZ_CP084413:c1394731-1394423 [Pseudomonas sp. HS-18]
MTYQLKQTAQFDHWLSELRAPRGKVAILRRLDRIRSGHFGDIRSVDIGVSEIRLDVGPGYRVYFTRRGDVPILLLCGGDK
TSQQRDIKLAQQLAGAIEWPLN

Nucleotide

• Length: 309 bp

>T218530 NZ_CP116035:737598-737894 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10565.04 Da
• Isoelectric Point: 4.6263

>AT218530 WP_037011255.1 NZ_CP084413:c1394438-1394142 [Pseudomonas sp. HS-18]
MAIELSDFDTSHHLDNEEVIAEYLSQVLSDGDTDELLLAIGNIAKARGMTKIANDSGLGRESLYKVFAPGAKPRFETVMK
VMRALGVELQAIPASSGK

Nucleotide

• Length: 297 bp

>AT218530 NZ_CP116035:737896-738291 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1B8TB76