Detailed information of TA system TA218529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1354658..1355369
• Replicon: chromosome
• Accession: NZ_CP084413
• Organism: Pseudomonas sp. HS-18

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LDJ84_RS06355
• Protein ID: WP_226046080.1
• Coordinates: 1354658..1355092 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: LDJ84_RS06360
• Protein ID: WP_226046081.1
• Coordinates: 1355097..1355369 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDJ84_RS06350 (LDJ84_06350)	1352578..1354620	+	2043	WP_065086149.1	exodeoxyribonuclease V subunit alpha	-
LDJ84_RS06355 (LDJ84_06355)	1354658..1355092	-	435	WP_226046080.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDJ84_RS06360 (LDJ84_06360)	1355097..1355369	-	273	WP_226046081.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LDJ84_RS06365 (LDJ84_06365)	1355503..1358349	+	2847	WP_065086150.1	ATP-binding protein	-
LDJ84_RS06370 (LDJ84_06370)	1358328..1359524	+	1197	WP_037008824.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-131)

Antitoxin

• SpoVT-AbrB domain (17-53)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15552.78 Da
• Isoelectric Point: 4.8185

>T218529 WP_226046080.1 NZ_CP084413:c1355092-1354658 [Pseudomonas sp. HS-18]
VATPVTYMLDTNICSFIMRQNPPQVLQRLQKAAESGNRLVISAITHAELRYGAASPKAPKAVAAWIDALVQRLDDVLPWD
IDAVDSSAKLMSLLLDSGTPIGPNDTGIASHALATDCVIVTNNTREFRRVPGLVVEDWCNSEPA

Nucleotide

• Length: 435 bp

>T218529 NZ_CP116035:673023-673337 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9917.17 Da
• Isoelectric Point: 4.2342

>AT218529 WP_226046081.1 NZ_CP084413:c1355369-1355097 [Pseudomonas sp. HS-18]
MLSDLEAPTPTTTVSLFRNGKNQAVRIPRDMEFKGVTELVMTREGDTLILRPSRPTWSSLAELEPVDADFLGDRPDVIAP
SRLGFGDDEG

Nucleotide

• Length: 273 bp

>AT218529 NZ_CP116035:673334-673750 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA