Detailed information of TA system TA218526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 237252..237829
• Replicon: plasmid pCFD-2-1
• Accession: NZ_CP084407
• Organism: Sphingobium sp. CFD-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LDB29_RS26535
• Protein ID: WP_257558133.1
• Coordinates: 237494..237829 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LDB29_RS26530
• Protein ID: WP_257558131.1
• Coordinates: 237252..237494 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB29_RS26500	232496..232762	+	267	Protein_222	transposase	-
LDB29_RS26505	233333..234034	-	702	WP_257558121.1	transposase	-
LDB29_RS26510	234034..234192	-	159	WP_257558124.1	hypothetical protein	-
LDB29_RS26515	234460..235317	-	858	WP_257558126.1	DUF3800 domain-containing protein	-
LDB29_RS26520	235963..236202	-	240	WP_257558128.1	type II toxin-antitoxin system ParD family antitoxin	-
LDB29_RS26525	236410..236655	+	246	Protein_227	integrase	-
LDB29_RS26530	237252..237494	+	243	WP_257558131.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LDB29_RS26535	237494..237829	+	336	WP_257558133.1	endoribonuclease MazF	Toxin
LDB29_RS26540	238146..238298	-	153	WP_257558135.1	hypothetical protein	-
LDB29_RS26545	239923..242718	+	2796	WP_257558137.1	UvrD-helicase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..360439	360439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-108)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12111.11 Da
• Isoelectric Point: 9.5822

>T218526 WP_257558133.1 NZ_CP084407:237494-237829 [Sphingobium sp. CFD-2]
MVSRYLPDTGDIVWLQFDPQAGHEQAGHRPALVLSPAAYNKTRGMMICCPMTSQIKGYPFEVVVSREPPSVVLSDQIKSL
DWKARAAKKKSSVSTAVIDEVRAKVGALLGI

Nucleotide

• Length: 336 bp

>T218526 NZ_CP116026:c1001542-1001201 [Enterococcus casseliflavus]
ATGATGAATGATCAAACTTACATTCCCAAAAAAGGGGATATCGTGTGGATCGATTTTGACCCTTCGGCTGGAAAGGAAAT
TCAAAAAAGACGTCCAGGATTAGTCGTCTCTCGCTATGAGTTTAATCGTAGAACGATGTTTGCGGTAATTTGTCCTATTA
CACATACAATTAGGAAATTTCCTACCCACTACACATTGCCAGATAGCATAGATACAGATGGACAAGTGATTATCTCTCAA
TTAAAATCACTAGACTTTAAAGAAAGAAAACTGAAAAAAATTGAAAACCTCCCTTTACAAGATATGGCGAAGATCGATCA
GATCATTGAGTATATTTTTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8587.96 Da
• Isoelectric Point: 4.6828

>AT218526 WP_257558131.1 NZ_CP084407:237252-237494 [Sphingobium sp. CFD-2]
MGVQVKKWGNSAAIRIPSAIMAAAALNVGQEVEVREEGGRLIIEPVLKPVYTLEGLLAGMKPETFHDNVDFGPPVGNEIF

Nucleotide

• Length: 243 bp

>AT218526 NZ_CP116026:c1001772-1001542 [Enterococcus casseliflavus]
ATGCTTATTACTAAATCAAGACTGCAAGGCAGTTCTGTCGTCATTACGCTTCCTTCTGATAACGGTAAGAAACCTTCTGA
AAATCAAGAATATGTTGTCGTCTACGCAGAGGATGGGACGATCATTCTTGTTCCTAAAATTGAAGATCCTTTTTATGGTG
GAGAAGAATCAGAGTTTTATGAAAAAGATGAGTGGGAAGACCTTTCACCTGAAGGGAGAGAAATTTTTTAA