Detailed information of TA system TA218524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 971033..971607
• Replicon: chromosome
• Accession: NZ_CP084406
• Organism: Sphingobium sp. CFD-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LDB29_RS24605
• Protein ID: WP_103001490.1
• Coordinates: 971033..971365 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LDB29_RS24610
• Protein ID: WP_082166021.1
• Coordinates: 971365..971607 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB29_RS24560	966317..967051	-	735	WP_257557350.1	zeta toxin family protein	-
LDB29_RS24565	967048..967368	-	321	WP_037481402.1	hypothetical protein	-
LDB29_RS24570	967427..967570	-	144	WP_257557352.1	hypothetical protein	-
LDB29_RS24575	967684..968388	-	705	WP_257558026.1	TonB-dependent receptor	-
LDB29_RS24580	968316..968717	-	402	WP_257558027.1	TonB-dependent receptor	-
LDB29_RS24585	968768..968926	-	159	WP_239659656.1	DUF736 domain-containing protein	-
LDB29_RS24590	969304..970260	+	957	WP_257557354.1	hypothetical protein	-
LDB29_RS24595	970292..970489	-	198	Protein_851	type II toxin-antitoxin system RelE/ParE family toxin	-
LDB29_RS24600	970489..970758	-	270	WP_044660855.1	type II toxin-antitoxin system ParD family antitoxin	-
LDB29_RS24605	971033..971365	-	333	WP_103001490.1	endoribonuclease MazF	Toxin
LDB29_RS24610	971365..971607	-	243	WP_082166021.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LDB29_RS24615	972309..972566	+	258	WP_048575602.1	addiction module antitoxin	-
LDB29_RS24620	972572..972886	+	315	WP_048575603.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDB29_RS24625	973197..973580	+	384	WP_041380262.1	transposase	-
LDB29_RS24630	973577..973924	+	348	WP_011950829.1	IS66 family insertion sequence element accessory protein TnpB	-
LDB29_RS24635	974027..975538	+	1512	WP_257557001.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	975801..976997	1196
	-	inside	Genomic island	-	-	968316..988238	19922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12070.22 Da
• Isoelectric Point: 9.9037

>T218524 WP_103001490.1 NZ_CP084406:c971365-971033 [Sphingobium sp. CFD-2]
MAKYVPDMGDIVWLNFDPQAGHEQAGHRPALVLSPAAYNRVRGMMICCPMTSKIKGYPFEVLTSQNLPSAVLADQLKSVD
WMTRKATKKGTVSQAALLEVQAKIRALLAI

Nucleotide

• Length: 333 bp

>T218524 NZ_CP116026:457415-457681 [Enterococcus casseliflavus]
ATGAGGAGTGATACGGTCACAATCAAAAACTCTGCGAAAGTGGATCTAAGGAAAATCAAACAATCGAATTTAAAAAAACA
GTTTGAAGAAGTGATTCAGGCTTTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATATTCACGTAGGCTGAATAGGCAACATCGAATGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCATGGACACATTATGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8799.10 Da
• Isoelectric Point: 4.5489

>AT218524 WP_082166021.1 NZ_CP084406:c971607-971365 [Sphingobium sp. CFD-2]
MRVRVKKWGNSASVRIPSSIMAAAALHIDQEVDVREEEGRVIIEPITAPAFTLEGLLAGMKPENFPENVDFGPPVGNELW

Nucleotide

• Length: 243 bp

>AT218524 NZ_CP116026:457152-457415 [Enterococcus casseliflavus]
ATGGCTAAAACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGATGTAAATGAAAACCATAC
TGAAATTGAGATTATTAGTGATCGAAGTGGAAACAATGCGGTGTTGATTGGGCTAGGAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTCTAGAAAAGGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGGATATCGAT
GATATTGATTGGCAAGCTCTTTGA