Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3104305..3104879 Replicon chromosome
Accession NZ_CP084405
Organism Sphingobium sp. CFD-2

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LDB29_RS15310 Protein ID WP_257554672.1
Coordinates 3104305..3104637 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag LDB29_RS15315 Protein ID WP_257554674.1
Coordinates 3104637..3104879 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB29_RS15275 3099468..3099779 - 312 WP_257554665.1 HU family DNA-binding protein -
LDB29_RS15280 3099906..3100610 - 705 WP_257554666.1 helix-turn-helix transcriptional regulator -
LDB29_RS15285 3100623..3100823 - 201 WP_257554667.1 hypothetical protein -
LDB29_RS15290 3100935..3101675 + 741 WP_257554668.1 energy transducer TonB -
LDB29_RS15295 3101728..3102279 + 552 WP_257554669.1 sigma-70 family RNA polymerase sigma factor -
LDB29_RS15300 3102342..3103337 + 996 WP_257554670.1 FecR domain-containing protein -
LDB29_RS15305 3103455..3104264 + 810 WP_257554671.1 helix-turn-helix transcriptional regulator -
LDB29_RS15310 3104305..3104637 - 333 WP_257554672.1 endoribonuclease MazF Toxin
LDB29_RS15315 3104637..3104879 - 243 WP_257554674.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LDB29_RS15320 3105054..3105887 - 834 WP_257554676.1 3-deoxy-manno-octulosonate cytidylyltransferase -
LDB29_RS15325 3105888..3106376 - 489 WP_257554678.1 CBS domain-containing protein -
LDB29_RS15330 3106518..3108014 + 1497 WP_082430708.1 IS21 family transposase -
LDB29_RS15335 3108011..3108778 + 768 WP_054944222.1 IS21-like element helper ATPase IstB -
LDB29_RS15340 3108850..3109386 - 537 WP_257554680.1 SIS domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3045126..3211387 166261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

Antitoxin PemI-like

(2-80)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12117.17 Da        Isoelectric Point: 9.6040

>T218521 WP_257554672.1 NZ_CP084405:c3104637-3104305 [Sphingobium sp. CFD-2]
MAAYVPDIGDIVWLEFDPQAGHEQAGHRPALVLSPEKYNGLRGMMICCPMTSRIKGYPFEVVESRTPPSVVLADQVKSLD
WRARRASKKGKISQAALAEVQAKIRALLAL

Download         Length: 333 bp

>T218521 NZ_CP116023:194113-194379 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCACCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8904.18 Da        Isoelectric Point: 4.3707

>AT218521 WP_257554674.1 NZ_CP084405:c3104879-3104637 [Sphingobium sp. CFD-2]
MMRVQVKKWGNSASVRIPASIMAAAALHIDQEVDVREEDGRIVIDPVMRSYSLDELLAKMTPDTFPDTIEFGAPVGNESW

Download         Length: 243 bp

>AT218521 NZ_CP116023:193850-194113 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
AATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References