Detailed information of TA system TA021852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 734129..734772
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: BCG_RS03335
• Protein ID: WP_003403187.1
• Coordinates: 734371..734772 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: BCG_RS03330
• Protein ID: WP_003403184.1
• Coordinates: 734129..734374 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS03295	729409..729939	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BCG_RS03300	729923..730618	-	696	WP_197044064.1	response regulator transcription factor	-
BCG_RS03305	730741..731052	+	312	WP_003403164.1	hypothetical protein	-
BCG_RS03310	731124..732074	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
BCG_RS03315	732315..732899	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
BCG_RS03320	732901..733614	+	714	Protein_659	IS607 family element transposase accessory protein TnpB	-
BCG_RS03325	733614..734084	+	471	WP_003898523.1	hypothetical protein	-
BCG_RS03330	734129..734374	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BCG_RS03335	734371..734772	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCG_RS21865	735160..735327	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
BCG_RS21870	735360..735557	-	198	WP_003403191.1	hypothetical protein	-
BCG_RS03350	735637..736794	-	1158	WP_003403193.1	hypothetical protein	-
BCG_RS03355	736846..737067	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
BCG_RS03360	737209..737814	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T21852 WP_003403187.1 NC_008769:734371-734772 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T21852 NC_008769:734371-734772 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT21852 WP_003403184.1 NC_008769:734129-734374 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT21852 NC_008769:734129-734374 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91