Detailed information of TA system TA218519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 2159215..2159672
• Replicon: chromosome
• Accession: NZ_CP084405
• Organism: Sphingobium sp. CFD-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LDB29_RS10520
• Protein ID: WP_099185663.1
• Coordinates: 2159397..2159672 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A292ZD49
• Locus tag: LDB29_RS10515
• Protein ID: WP_044662618.1
• Coordinates: 2159215..2159397 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB29_RS10495	2154333..2157245	+	2913	WP_257554093.1	excinuclease ABC subunit UvrA	-
LDB29_RS10500	2157321..2158397	+	1077	WP_044662616.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
LDB29_RS10505	2158405..2158965	+	561	WP_099185665.1	hypothetical protein	-
LDB29_RS10510	2158949..2159137	+	189	WP_257554095.1	hypothetical protein	-
LDB29_RS10515	2159215..2159397	+	183	WP_044662618.1	hypothetical protein	Antitoxin
LDB29_RS10520	2159397..2159672	+	276	WP_099185663.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDB29_RS10525	2159756..2160763	+	1008	WP_099185662.1	hypothetical protein	-
LDB29_RS10530	2160934..2161146	+	213	WP_007684722.1	cold-shock protein	-
LDB29_RS10535	2161209..2161385	+	177	WP_165363300.1	hypothetical protein	-
LDB29_RS10540	2161514..2161732	+	219	WP_025550213.1	hypothetical protein	-
LDB29_RS10545	2161903..2162247	+	345	WP_025550214.1	glycine zipper 2TM domain-containing protein	-
LDB29_RS10550	2162305..2163237	-	933	WP_025550215.1	FAD-binding protein	-
LDB29_RS10555	2163234..2163983	-	750	WP_025550216.1	electron transfer flavoprotein subunit beta/FixA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10410.87 Da
• Isoelectric Point: 6.4801

>T218519 WP_099185663.1 NZ_CP084405:2159397-2159672 [Sphingobium sp. CFD-2]
MPHVIWRPKALEDADRIIDYISDRNPAAAARLADLFEYAAERLADHPYMHRAGRVPDTREAIVTPNYVLVYRAGADVIEI
LAVLHTRQQYP

Nucleotide

• Length: 276 bp

>T218519 NZ_CP116014:500430-500780 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6641.62 Da
• Isoelectric Point: 5.7833

>AT218519 WP_044662618.1 NZ_CP084405:2159215-2159397 [Sphingobium sp. CFD-2]
MTKLTPIESEFATTEDAEAYDAWFRAKVEAALADPRPGIPHDQVMAKLRAIIEAKKAKQA

Nucleotide

• Length: 183 bp

>AT218519 NZ_CP116014:500143-500424 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCACAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAACATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A292ZD49