Detailed information of TA system TA218516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 72440..72971
• Replicon: plasmid pCFD-1-1
• Accession: NZ_CP084399
• Organism: Sphingobium sp. CFD-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: LDB26_RS19700
• Protein ID: WP_004212690.1
• Coordinates: 72690..72971 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: LDB26_RS19695
• Protein ID: WP_004212689.1
• Coordinates: 72440..72703 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB26_RS19675	67546..68112	+	567	WP_008786762.1	TetR/AcrR family transcriptional regulator	-
LDB26_RS19680	68255..70708	+	2454	WP_257542281.1	Tn3 family transposase	-
LDB26_RS19685	70727..71491	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
LDB26_RS19690	71618..72055	+	438	WP_007683347.1	DUF411 domain-containing protein	-
LDB26_RS19695	72440..72703	+	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LDB26_RS19700	72690..72971	+	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LDB26_RS19705	73319..73687	+	369	WP_239659617.1	CorA family divalent cation transporter	-
LDB26_RS19710	73750..74157	-	408	WP_007683354.1	response regulator	-
LDB26_RS19715	74239..74862	-	624	WP_007683357.1	response regulator	-
LDB26_RS19720	74852..76387	-	1536	WP_007683359.1	PAS domain S-box protein	-
LDB26_RS19725	76446..76919	-	474	WP_007683361.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..160984	160984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T218516 WP_004212690.1 NZ_CP084399:72690-72971 [Sphingobium sp. CFD-1]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T218516 NZ_CP116012:c3789119-3788769 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT218516 WP_004212689.1 NZ_CP084399:72440-72703 [Sphingobium sp. CFD-1]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT218516 NZ_CP116012:c3789405-3789124 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968