Detailed information of TA system TA218515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2879928..2880487
• Replicon: chromosome
• Accession: NZ_CP084398
• Organism: Sphingobium sp. CFD-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LDB26_RS14545
• Protein ID: WP_257538963.1
• Coordinates: 2880194..2880487 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LDB26_RS14540
• Protein ID: WP_257538961.1
• Coordinates: 2879928..2880197 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB26_RS14520	2875259..2876281	-	1023	WP_257538943.1	P-type conjugative transfer ATPase TrbB	-
LDB26_RS14525	2876278..2876694	-	417	WP_257538945.1	CopG family transcriptional regulator	-
LDB26_RS14530	2876691..2878712	-	2022	WP_257538959.1	conjugal transfer protein TraG	-
LDB26_RS14535	2878858..2879778	-	921	WP_257538960.1	HEPN domain-containing protein	-
LDB26_RS14540	2879928..2880197	+	270	WP_257538961.1	ribbon-helix-helix domain-containing protein	Antitoxin
LDB26_RS14545	2880194..2880487	+	294	WP_257538963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDB26_RS14550	2880583..2882595	-	2013	WP_257538965.1	relaxase/mobilization nuclease RlxS	-
LDB26_RS14555	2882889..2883095	+	207	WP_257538966.1	hypothetical protein	-
LDB26_RS14560	2883146..2883445	-	300	WP_257538967.1	hypothetical protein	-
LDB26_RS14565	2883760..2884449	-	690	WP_257538968.1	pentapeptide repeat-containing protein	-
LDB26_RS14570	2884556..2885191	-	636	WP_257538969.1	lytic transglycosylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2858212..2927515	69303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11527.27 Da
• Isoelectric Point: 8.1243

>T218515 WP_257538963.1 NZ_CP084398:2880194-2880487 [Sphingobium sp. CFD-1]
MRIQWTSKASSDLLRLHEHLRPVAPEAAARIVQQLAHAPDRLLDYPRLGEKLEAFEPREVRRIIVGHYEMRYEIVSGTIF
ILRLWHCRENRSFEAEH

Nucleotide

• Length: 294 bp

>T218515 NZ_CP116012:2959268-2960014 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATATACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9605.86 Da
• Isoelectric Point: 5.0484

>AT218515 WP_257538961.1 NZ_CP084398:2879928-2880197 [Sphingobium sp. CFD-1]
MAAGTRVLTAHVPEGMADKVDALAARIERSRGWIMKQALAAWIDQEEERHKMTLEALADVDAGRVIDHQSIQAWADSLST
GSPLPSPSA

Nucleotide

• Length: 270 bp

>AT218515 NZ_CP116012:2960014-2960184 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAGAGCATGA