Detailed information of TA system TA218514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2859951..2860516
• Replicon: chromosome
• Accession: NZ_CP084398
• Organism: Sphingobium sp. CFD-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D4Z593
• Locus tag: LDB26_RS14435
• Protein ID: WP_013041067.1
• Coordinates: 2860145..2860516 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LDB26_RS14430
• Protein ID: WP_013041066.1
• Coordinates: 2859951..2860148 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB26_RS14400	2854976..2855191	-	216	WP_257538914.1	hypothetical protein	-
LDB26_RS14405	2855305..2856198	-	894	WP_257538916.1	N-formylglutamate amidohydrolase	-
LDB26_RS14410	2856338..2856715	+	378	WP_257538918.1	response regulator	-
LDB26_RS14415	2856721..2858052	-	1332	WP_257538921.1	dicarboxylate/amino acid:cation symporter	-
LDB26_RS14425	2858764..2859801	+	1038	WP_257538923.1	site-specific integrase	-
LDB26_RS14430	2859951..2860148	+	198	WP_013041066.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LDB26_RS14435	2860145..2860516	+	372	WP_013041067.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDB26_RS14440	2860881..2861294	-	414	WP_257538924.1	transposase	-
LDB26_RS14445	2861800..2863653	-	1854	WP_257538925.1	HipA domain-containing protein	-
LDB26_RS14450	2863843..2864998	-	1156	Protein_2842	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2858212..2927515	69303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13809.98 Da
• Isoelectric Point: 6.4909

>T218514 WP_013041067.1 NZ_CP084398:2860145-2860516 [Sphingobium sp. CFD-1]
MILVDTSVWIDHLRHDDPVLSQSLSKRQVLSHPFIIGELALGSLRQRDLILEALRGLPSVIVAHDEEVHAFIDLHRLFGI
GIGYIDAHLLAATLLTPDVQFWTRDKRLRAAALRLGVDANLDH

Nucleotide

• Length: 372 bp

>T218514 NZ_CP116012:c2374070-2373894 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7069.07 Da
• Isoelectric Point: 8.9168

>AT218514 WP_013041066.1 NZ_CP084398:2859951-2860148 [Sphingobium sp. CFD-1]
MRTTVALDDELVAKAQAFTGLTEKSSLVREALKALIERESSARLVRLGGTEPHGEAAPRRRPDPV

Nucleotide

• Length: 198 bp

>AT218514 NZ_CP116012:2374011-2374111 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTACGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Structures

Toxin

• AlphaFold DB: D4Z593