Detailed information of TA system TA218512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1564955..1565724
• Replicon: chromosome
• Accession: NZ_CP084398
• Organism: Sphingobium sp. CFD-1

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A243PD42
• Locus tag: LDB26_RS08110
• Protein ID: WP_048577672.1
• Coordinates: 1565197..1565724 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: LDB26_RS08105
• Protein ID: WP_237449632.1
• Coordinates: 1564955..1565200 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDB26_RS08080	1560686..1561453	+	768	WP_063976957.1	IS21-like element helper ATPase IstB	-
LDB26_RS08085	1561579..1562175	-	597	Protein_1589	MBL fold metallo-hydrolase	-
LDB26_RS08090	1562409..1563341	+	933	WP_048578303.1	1-phosphofructokinase family hexose kinase	-
LDB26_RS08095	1563533..1563691	+	159	WP_158500997.1	hypothetical protein	-
LDB26_RS08100	1563809..1564687	+	879	WP_202408155.1	LuxR family transcriptional regulator	-
LDB26_RS08105	1564955..1565200	+	246	WP_237449632.1	DUF1778 domain-containing protein	Antitoxin
LDB26_RS08110	1565197..1565724	+	528	WP_048577672.1	hypothetical protein	Toxin
LDB26_RS08115	1565968..1566423	+	456	WP_048577673.1	hypothetical protein	-
LDB26_RS08120	1566410..1570180	-	3771	WP_048577674.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA / katA	1450428..1586240	135812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-78)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19131.69 Da
• Isoelectric Point: 6.7528

>T218512 WP_048577672.1 NZ_CP084398:1565197-1565724 [Sphingobium sp. CFD-1]
MTLPSWHEEAISKAHDRTSFDCGDNDLNVYLQRYARQSHEAGGAKTFLAIDDTDSKTVLGFYSIAPGSMDHADTPELARR
GLARHEVPGFRLARLATDLRVQGQGLGGQLLGAIGRRCIRVATEVGGVMLIIDAKNERAADWYAGYGAIPLHDKPLTLIL
PFATLERELRAAGQF

Nucleotide

• Length: 528 bp

>T218512 NZ_CP116012:c2373875-2373624 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGTAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATTAAGGATTACTTCAGCAGCGAAGCACAAACACTAA
TCAATATAATATTCATAGTCACATGTATCATTGCCATATTGTTATGGATTATCTATTTTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8974.49 Da
• Isoelectric Point: 10.1783

>AT218512 WP_237449632.1 NZ_CP084398:1564955-1565200 [Sphingobium sp. CFD-1]
MSLRIRPEQKALLARAAALKHTDLTTFVTRSALREAENVIEESERTKLSERDSLLVLDLLENPPPVNAKLRAAIAAMPKL
A

Nucleotide

• Length: 246 bp

>AT218512 NZ_CP116012:2373853-2373953 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Structures

Toxin

• AlphaFold DB: A0A243PD42