Detailed information of TA system TA021851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 728039..728685
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: BCG_RS03280
• Protein ID: WP_003403137.1
• Coordinates: 728039..728452 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: BCG_RS03285
• Protein ID: WP_003403139.1
• Coordinates: 728449..728685 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS03260	724122..725672	+	1551	WP_003403119.1	MCE family protein	-
BCG_RS03265	725724..726116	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
BCG_RS03270	726113..726370	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
BCG_RS03275	726553..727788	-	1236	WP_003403128.1	ATP-binding protein	-
BCG_RS03280	728039..728452	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCG_RS03285	728449..728685	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BCG_RS03290	728789..729295	-	507	WP_003900973.1	two component system sensor kinase HK1	-
BCG_RS03295	729409..729939	-	531	WP_003917320.1	two component system sensor kinase HK2	-
BCG_RS03300	729923..730618	-	696	WP_197044064.1	response regulator transcription factor	-
BCG_RS03305	730741..731052	+	312	WP_003403164.1	hypothetical protein	-
BCG_RS03310	731124..732074	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
BCG_RS03315	732315..732899	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
BCG_RS03320	732901..733614	+	714	Protein_659	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T21851 WP_003403137.1 NC_008769:c728452-728039 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T21851 NC_008769:c728452-728039 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT21851 WP_003403139.1 NC_008769:c728685-728449 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT21851 NC_008769:c728685-728449 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89