Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5325230..5325855 Replicon chromosome
Accession NZ_CP084397
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KF608_RS28690 Protein ID WP_002882817.1
Coordinates 5325230..5325613 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KF608_RS28695 Protein ID WP_004150355.1
Coordinates 5325613..5325855 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF608_RS28675 (5322596) 5322596..5323498 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KF608_RS28680 (5323495) 5323495..5324130 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KF608_RS28685 (5324127) 5324127..5325056 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KF608_RS28690 (5325230) 5325230..5325613 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KF608_RS28695 (5325613) 5325613..5325855 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KF608_RS28700 (5326060) 5326060..5326977 + 918 WP_021466676.1 alpha/beta hydrolase -
KF608_RS28705 (5326991) 5326991..5327932 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KF608_RS28710 (5327977) 5327977..5328414 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KF608_RS28715 (5328411) 5328411..5329271 - 861 WP_004146232.1 virulence factor BrkB family protein -
KF608_RS28720 (5329265) 5329265..5329864 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218509 WP_002882817.1 NZ_CP084397:c5325613-5325230 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218509 NZ_CP116010:c3548652-3548302 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218509 WP_004150355.1 NZ_CP084397:c5325855-5325613 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218509 NZ_CP116010:c3548939-3548658 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References