Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4731206..4732016 Replicon chromosome
Accession NZ_CP084397
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KF608_RS25885 Protein ID WP_002887280.1
Coordinates 4731206..4731739 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KF608_RS25890 Protein ID WP_002887278.1
Coordinates 4731750..4732016 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF608_RS25880 (4730038) 4730038..4731159 + 1122 WP_009309849.1 cupin domain-containing protein -
KF608_RS25885 (4731206) 4731206..4731739 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KF608_RS25890 (4731750) 4731750..4732016 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KF608_RS25895 (4732119) 4732119..4733552 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
KF608_RS25900 (4733542) 4733542..4734225 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
KF608_RS25905 (4734398) 4734398..4735783 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
KF608_RS25910 (4735801) 4735801..4736106 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T218506 WP_002887280.1 NZ_CP084397:c4731739-4731206 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T218506 NZ_CP116007:c1065587-1065393 [Pseudoxanthomonas mexicana]
GTGCCTCCGAAGATTCGCGATGTATTGCAGCAGCTGAAGGACGCAGGCTGGCAGCAGGTCGGCCAGACAGGCAGCCGCCG
CCAGTTCAAGCATCCAACGCTGCCGGGCCGGGTGACAGTCGCCGGCAAGCCGGGTGATGATGTGGCGCCAGGCACGTTGG
GCAGCATCCTGAAACAGGCCGGGATCAAGAGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT218506 WP_002887278.1 NZ_CP084397:c4732016-4731750 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT218506 NZ_CP116007:c1065387-1065175 [Pseudoxanthomonas mexicana]
ATGCGCTACGCCATCGTCATCGAGCAAGCGGGCGGCAACTATTCCGCCTATGTGCCGGACCTGCCTGGCTGCGTGGCCAC
CGGTGCGACCAAGGCCGAGGTGGAAGCGTTGATCCACGACGCCATTGCCTTGCATCTCGCGGGAATGCGCGAGGATGGGT
TGGCGATACCGATGCCGTCGAGCCAGGTGGAGTACATCGACGTCGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References