Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4103372..4103991 Replicon chromosome
Accession NZ_CP084397
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KF608_RS22895 Protein ID WP_002892050.1
Coordinates 4103773..4103991 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KF608_RS22890 Protein ID WP_002892066.1
Coordinates 4103372..4103746 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF608_RS22880 (4098524) 4098524..4099717 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KF608_RS22885 (4099740) 4099740..4102886 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KF608_RS22890 (4103372) 4103372..4103746 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KF608_RS22895 (4103773) 4103773..4103991 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KF608_RS22900 (4104154) 4104154..4104720 + 567 WP_002892030.1 maltose O-acetyltransferase -
KF608_RS22905 (4104692) 4104692..4104832 - 141 WP_004147370.1 hypothetical protein -
KF608_RS22910 (4104853) 4104853..4105323 + 471 WP_020802585.1 YlaC family protein -
KF608_RS22915 (4105298) 4105298..4106749 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
KF608_RS22920 (4106850) 4106850..4107548 + 699 WP_023287311.1 GNAT family protein -
KF608_RS22925 (4107545) 4107545..4107685 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KF608_RS22930 (4107685) 4107685..4107948 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218505 WP_002892050.1 NZ_CP084397:4103773-4103991 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T218505 NZ_CP116006:2867278-2867454 [Achromobacter mucicolens]
GTGAAGCAAAGCGAGTTCAAACGGTGGCTCGCTTCCAAAGGAGTGACTTTCGCAGAGGGTAGCAACCACACGAAAGCCTA
CTACAACGGAAAACAGACCACCCTGCCTAGGCACCCTTCCAAGGAATTGAGAGACGGCACTAGGCAGAACATCCTGAAGA
AACTAGGACTGAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT218505 WP_002892066.1 NZ_CP084397:4103372-4103746 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT218505 NZ_CP116006:2867511-2867933 [Achromobacter mucicolens]
ATGGCTCGATATCCTGCATTTCTCGACCCCGAGGGCGACGGCTTCAACGTTACGTTCCGTGATATCCCGGAGGCGATCAC
CTGCGGCGACAACCGCGATGACGCGCTGGAAATGGCCGCCGGCGCTCTGGTGACTGCCATGGAGTTCTATTTTGAGGACC
GTCGGCCCGTCCCTCTTCCGTCCGATCCCAAAGATGGAGAGGTTCTTATCGCCCTTCCCGCGAGCGTATGGGCGAAGGTT
CTATTGCTGAACGAGATGATCGCTCAGCGTGTCACGCCTGCAGAGTTGGCCCGCAGGTTGAACACACGCCCGCAAGAGGT
GACCCGCATCATAAATCTGGGTCATGCCACGAAGATCGACACCATCGCGGCGGCCTTGCGCACGATGGGAAAGGATCTCG
AGATTTCCGTCCGACAAGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References