Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 725724..726370 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag BCG_RS03265 Protein ID WP_003403122.1
Coordinates 725724..726116 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag BCG_RS03270 Protein ID WP_003403125.1
Coordinates 726113..726370 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS03250 721384..722912 + 1529 Protein_645 virulence factor Mce family protein -
BCG_RS03255 722975..724117 + 1143 WP_003911253.1 virulence factor Mce family protein -
BCG_RS03260 724122..725672 + 1551 WP_003403119.1 MCE family protein -
BCG_RS03265 725724..726116 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
BCG_RS03270 726113..726370 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
BCG_RS03275 726553..727788 - 1236 WP_003403128.1 ATP-binding protein -
BCG_RS03280 728039..728452 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
BCG_RS03285 728449..728685 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BCG_RS03290 728789..729295 - 507 WP_003900973.1 two component system sensor kinase HK1 -
BCG_RS03295 729409..729939 - 531 WP_003917320.1 two component system sensor kinase HK2 -
BCG_RS03300 729923..730618 - 696 WP_197044064.1 response regulator transcription factor -
BCG_RS03305 730741..731052 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T21850 WP_003403122.1 NC_008769:c726116-725724 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T21850 NC_008769:c726116-725724 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT21850 WP_003403125.1 NC_008769:c726370-726113 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT21850 NC_008769:c726370-726113 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References