Detailed information of TA system TA218495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1468874..1469610
• Replicon: chromosome
• Accession: NZ_CP084397
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: KF608_RS09880
• Protein ID: WP_032433360.1
• Coordinates: 1469128..1469610 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KF608_RS09875
• Protein ID: WP_003026799.1
• Coordinates: 1468874..1469140 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF608_RS09850 (1464520)	1464520..1465659	+	1140	WP_032433368.1	mannitol dehydrogenase	-
KF608_RS09855 (1465688)	1465688..1466350	+	663	WP_032433366.1	dihydroxyacetone kinase subunit DhaL	-
KF608_RS09860 (1466334)	1466334..1467338	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
KF608_RS09865 (1467356)	1467356..1467988	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
KF608_RS09870 (1467998)	1467998..1468561	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
KF608_RS09875 (1468874)	1468874..1469140	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KF608_RS09880 (1469128)	1469128..1469610	+	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
KF608_RS09885 (1469810)	1469810..1471213	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
KF608_RS09890 (1471242)	1471242..1471874	-	633	WP_001567369.1	hypothetical protein	-
KF608_RS09895 (1472254)	1472254..1472853	-	600	WP_064472304.1	helix-turn-helix transcriptional regulator	-
KF608_RS09900 (1473066)	1473066..1474010	-	945	WP_077254249.1	fimbrial protein	-
KF608_RS09905 (1474022)	1474022..1474600	-	579	WP_032432061.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1436819..1482836	46017
	-	flank	IS/Tn	-	-	1469810..1471213	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T218495 WP_032433360.1 NZ_CP084397:1469128-1469610 [Klebsiella pneumoniae subsp. pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T218495 NZ_CP116004:c1092316-1092113 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT218495 WP_003026799.1 NZ_CP084397:1468874-1469140 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT218495 NZ_CP116004:c1092705-1092337 [Edwardsiella piscicida]
ATGGATGAGTACACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAATCTACAGCTTAATGAGCTGATTGAGC
ATATTGCCAGCGTCGCGCAATCATTTAAGATCAAATATCCGCATCATTCGGATTTAGCCGAAATATTGGATGATTATCTG
GATGAAACCTATGCCCTGTTTGGCGCCTATAGCGTCAGTGAAACGGCGCTCCGGCACTGGCTGCGCACCAAGCGGCGGGT
GGCATACTGCCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66