Detailed information of TA system TA218490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 293311..293833
• Replicon: plasmid pSI646A-ARMA-Vir-NDM
• Accession: NZ_CP084395
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: KF608_RS02210
• Protein ID: WP_004181778.1
• Coordinates: 293549..293833 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: KF608_RS02205
• Protein ID: WP_025368601.1
• Coordinates: 293311..293559 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF608_RS02185 (KF608_02185)	288520..289356	-	837	WP_223686625.1	hypothetical protein	-
KF608_RS02190 (KF608_02190)	289403..289756	-	354	WP_004181774.1	hypothetical protein	-
KF608_RS02195 (KF608_02195)	289901..290887	-	987	WP_025368599.1	hypothetical protein	-
KF608_RS02200 (KF608_02200)	291221..293020	-	1800	WP_025368600.1	ATP-dependent helicase	-
KF608_RS02205 (KF608_02205)	293311..293559	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KF608_RS02210 (KF608_02210)	293549..293833	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF608_RS02215 (KF608_02215)	294063..295289	+	1227	WP_025368602.1	RNA-guided endonuclease TnpB family protein	-
KF608_RS02220 (KF608_02220)	295575..296000	-	426	WP_040120489.1	IS200/IS605 family transposase	-
KF608_RS02225 (KF608_02225)	296037..297263	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
KF608_RS02230 (KF608_02230)	297329..298455	-	1127	Protein_328	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaNDM-1 / aph(3')-VI / qnrS1 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..374716	374716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T218490 WP_004181778.1 NZ_CP084395:293549-293833 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T218490 NZ_CP116003:c1714809-1714528 [Edwardsiella piscicida]
ATGACCTATAAACTGAGCTTTGAAAAGCGAGCCCTGAAAGAGTGGAAAAAACTGGCACCGCCCATTCAAAGCCAGTTGAA
AAAGAAACTGATCGAACGCCTAGAAAATCCACATATCCCGGCCGCCCGCTTAAGTGGGCGCGCTAACCGTTATAAAATAA
AATTACGATCTTCCGGTTATCGGTTAGTTTATGAAGTCAACGATAGCGAAATCATCCTGCTGGTGATCGCTATCGGTAAA
CGAGCCGATAACGACGTGTACCTAGCGGCAGACGGTCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT218490 WP_025368601.1 NZ_CP084395:293311-293559 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT218490 NZ_CP116003:c1715050-1714799 [Edwardsiella piscicida]
ATGGCATACCAAATTCTCACTACAACAGCCGCCAGTATTACCGATCTAAAACGCAATCCGATGGGTACCATTGCTGAGGG
TGACGGCAATGCCGTCGCAATCTTAAACCGGAATGAGCCGGCCTTTTACTGCGTTCCACCGGAGCTCTATGCTTACTACC
TGGAGCTGGCTGAAAATGCTGCTCTTAATCGTATCGCCGATGAGCGTCTAGAGGACGCTGAATTCGTCAGCGTAAATATC
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53