218488

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 37236..38146 Replicon plasmid pSI646A-ARMA-Vir-NDM
Accession NZ_CP084395
Organism Klebsiella pneumoniae subsp. pneumoniae strain MRSN752019

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KF608_RS00830 Protein ID WP_004026354.1
Coordinates 37676..38146 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KF608_RS00825 Protein ID WP_004181895.1
Coordinates 37236..37679 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF608_RS00810 (KF608_00810) 33869..35356 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KF608_RS00815 (KF608_00815) 35442..35864 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KF608_RS00820 (KF608_00820) 35864..37135 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KF608_RS00825 (KF608_00825) 37236..37679 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KF608_RS00830 (KF608_00830) 37676..38146 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KF608_RS00835 (KF608_00835) 38256..38516 - 261 WP_004026352.1 hypothetical protein -
KF608_RS29120 39238..39396 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
KF608_RS00850 (KF608_00850) 39999..40973 + 975 WP_058842863.1 hypothetical protein -
KF608_RS29130 40957..41079 + 123 WP_257183836.1 hypothetical protein -
KF608_RS00855 (KF608_00855) 41171..41548 - 378 WP_029884711.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaNDM-1 / aph(3')-VI / qnrS1 / blaCTX-M-15 rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..374716 374716


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T218488 WP_004026354.1 NZ_CP084395:37676-38146 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T218488 NZ_CP115994:c105125-104262 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACACCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGCACAGGACGAACAACAGACGTTCCTATTCAAACAGCCACAATGCTACAAG
CCAAAGGTTATGAAACAAAAACGTACGCCATGGCAGTACCCAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT218488 WP_004181895.1 NZ_CP084395:37236-37679 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT218488 NZ_CP115994:c105399-105127 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References