218486

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 63595..64106 Replicon plasmid pIBH002-1
Accession NZ_CP084390
Organism Lactobacillus helsingborgensis strain IBH002

Toxin (Protein)


Gene name hicA Uniprot ID A0A0F4LPU4
Locus tag LDX53_RS09165 Protein ID WP_046325857.1
Coordinates 63855..64106 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0F4LQM4
Locus tag LDX53_RS09160 Protein ID WP_046325856.1
Coordinates 63595..63858 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDX53_RS09125 (LDX53_09135) 59311..59400 - 90 WP_244885273.1 putative holin-like toxin -
LDX53_RS09130 (LDX53_09140) 60057..60926 - 870 WP_046327892.1 hypothetical protein -
LDX53_RS09135 (LDX53_09145) 60984..61841 - 858 WP_046327893.1 hypothetical protein -
LDX53_RS09140 (LDX53_09150) 61831..62406 - 576 WP_052727806.1 JAB domain-containing protein -
LDX53_RS09145 (LDX53_09155) 62433..62648 - 216 WP_046325853.1 hypothetical protein -
LDX53_RS09150 (LDX53_09160) 62663..62980 - 318 WP_046325854.1 hypothetical protein -
LDX53_RS09155 (LDX53_09165) 62994..63515 - 522 WP_046325855.1 hypothetical protein -
LDX53_RS09160 (LDX53_09170) 63595..63858 - 264 WP_046325856.1 hypothetical protein Antitoxin
LDX53_RS09165 (LDX53_09175) 63855..64106 - 252 WP_046325857.1 type II toxin-antitoxin system HicA family toxin Toxin
LDX53_RS09170 (LDX53_09180) 64103..65638 - 1536 WP_046325858.1 DUF3854 domain-containing protein -
LDX53_RS09175 (LDX53_09185) 65976..66923 - 948 WP_257011528.1 class A sortase -
LDX53_RS09180 (LDX53_09190) 66886..67212 - 327 WP_046325860.1 hypothetical protein -
LDX53_RS09185 (LDX53_09195) 67436..68002 - 567 WP_046327894.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..124670 124670


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(21-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9878.45 Da        Isoelectric Point: 10.2925

>T218486 WP_046325857.1 NZ_CP084390:c64106-63855 [Lactobacillus helsingborgensis]
MNKYKKLLERFNSKPNDFTFEEAKKLLSYSDFKLVMKGHSSGSRVQFRCDNVKIDLHRPHPKNYLKKYQLEDIEESLKKA
GKL

Download         Length: 252 bp

>T218486 NZ_CP115994:22722-23063 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATAGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9846.90 Da        Isoelectric Point: 4.1473

>AT218486 WP_046325856.1 NZ_CP084390:c63858-63595 [Lactobacillus helsingborgensis]
MSLLSYKGYTGSIEFSAEDNIMYGRVLGLKNSIISYEGKSFQELENDFKDGINDYLASCIAEHEQPETSSKDSVRKDWNV
LLDQELA

Download         Length: 264 bp

>AT218486 NZ_CP115994:22492-22722 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F4LPU4

Antitoxin

Source ID Structure
AlphaFold DB A0A0F4LQM4

References