Detailed information of TA system TA218485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1701004..1701581
• Replicon: chromosome
• Accession: NZ_CP084389
• Organism: Lactobacillus helsingborgensis strain IBH002

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F4LWA8
• Locus tag: LDX53_RS08055
• Protein ID: WP_046327719.1
• Coordinates: 1701249..1701581 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LDX53_RS08050
• Protein ID: WP_052727785.1
• Coordinates: 1701004..1701255 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDX53_RS08040 (LDX53_08060)	1698530..1699459	-	930	WP_046327716.1	hypothetical protein	-
LDX53_RS08045 (LDX53_08065)	1699609..1700847	+	1239	WP_046327718.1	MFS transporter	-
LDX53_RS08050 (LDX53_08070)	1701004..1701255	+	252	WP_052727785.1	hypothetical protein	Antitoxin
LDX53_RS08055 (LDX53_08075)	1701249..1701581	+	333	WP_046327719.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LDX53_RS08060	1701668..1701799	+	132	WP_257011517.1	hypothetical protein	-
LDX53_RS08065 (LDX53_08080)	1701864..1702766	-	903	WP_046327720.1	LysR family transcriptional regulator	-
LDX53_RS08070 (LDX53_08085)	1702872..1704113	+	1242	WP_046327721.1	MFS transporter	-
LDX53_RS08075 (LDX53_08090)	1704118..1705557	+	1440	WP_046327722.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12781.92 Da
• Isoelectric Point: 9.4243

>T218485 WP_046327719.1 NZ_CP084389:1701249-1701581 [Lactobacillus helsingborgensis]
MVIKQGSILLMDLSPKQGHEQSGKRPYLYLSYKGVEKYAHIAVFAPISTTKRRYPLYQELPAVSQTKGVVMLDQLVTIDY
HNQSFQYLEQLPDDYMEKILKIVKVVFQKD

Nucleotide

• Length: 333 bp

>T218485 NZ_CP115993:c26851-26489 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9595.74 Da
• Isoelectric Point: 4.4992

>AT218485 WP_052727785.1 NZ_CP084389:1701004-1701255 [Lactobacillus helsingborgensis]
MIRATISKWGNSKGIRLNQAVLQALNATIGDQLNFKIEDNKVIMSKQDDSTDLTFEELFKDYSGEEFDTEIKLPDERKGN
ERW

Nucleotide

• Length: 252 bp

>AT218485 NZ_CP115993:c27108-26845 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTACATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0F4LWA8