Detailed information of TA system TA218484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 116774..117285
• Replicon: plasmid pIBH003-1
• Accession: NZ_CP084385
• Organism: Lactobacillus helsingborgensis strain IBH003

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LDX52_RS09645
• Protein ID: WP_267271859.1
• Coordinates: 116774..117025 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0F4LQM4
• Locus tag: LDX52_RS09650
• Protein ID: WP_046325856.1
• Coordinates: 117022..117285 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDX52_RS09625 (LDX52_09610)	112878..113444	+	567	WP_267271856.1	hypothetical protein	-
LDX52_RS09630 (LDX52_09615)	113668..113994	+	327	WP_267271857.1	hypothetical protein	-
LDX52_RS09635 (LDX52_09620)	114032..114904	+	873	WP_267271906.1	class A sortase	-
LDX52_RS09640 (LDX52_09625)	115242..116777	+	1536	WP_267271858.1	DUF3854 domain-containing protein	-
LDX52_RS09645 (LDX52_09630)	116774..117025	+	252	WP_267271859.1	type II toxin-antitoxin system HicA family toxin	Toxin
LDX52_RS09650 (LDX52_09635)	117022..117285	+	264	WP_046325856.1	hypothetical protein	Antitoxin
LDX52_RS09655 (LDX52_09640)	117365..117886	+	522	WP_267271860.1	hypothetical protein	-
LDX52_RS09660 (LDX52_09645)	117900..118217	+	318	WP_267271861.1	hypothetical protein	-
LDX52_RS09665 (LDX52_09650)	118232..118447	+	216	WP_267271862.1	hypothetical protein	-
LDX52_RS09670 (LDX52_09655)	118474..119049	+	576	WP_052724694.1	JAB domain-containing protein	-
LDX52_RS09675 (LDX52_09660)	119039..119923	+	885	WP_046325852.1	hypothetical protein	-
LDX52_RS09680 (LDX52_09665)	119981..120850	+	870	WP_046325851.1	hypothetical protein	-
LDX52_RS09685 (LDX52_09670)	121507..121596	+	90	WP_244885273.1	putative holin-like toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131542	131542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (21-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9848.43 Da
• Isoelectric Point: 10.2925

>T218484 WP_267271859.1 NZ_CP084385:116774-117025 [Lactobacillus helsingborgensis]
MNKYKKLLERFNSKPNDFTFEEAKKLLSYSDFKLVMKGHSSGSRVQFRCDNVKIDLHRPHPKNYLKKYQLEDIEEGLKKA
GKL

Nucleotide

• Length: 252 bp

>T218484 NZ_CP115993:8398-8739 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9846.90 Da
• Isoelectric Point: 4.1473

>AT218484 WP_046325856.1 NZ_CP084385:117022-117285 [Lactobacillus helsingborgensis]
MSLLSYKGYTGSIEFSAEDNIMYGRVLGLKNSIISYEGKSFQELENDFKDGINDYLASCIAEHEQPETSSKDSVRKDWNV
LLDQELA

Nucleotide

• Length: 264 bp

>AT218484 NZ_CP115993:8168-8398 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F4LQM4