Detailed information of TA system TA218483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1740899..1741476
• Replicon: chromosome
• Accession: NZ_CP084384
• Organism: Lactobacillus helsingborgensis strain IBH003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LDX52_RS08345
• Protein ID: WP_267271220.1
• Coordinates: 1741144..1741476 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LDX52_RS08340
• Protein ID: WP_109918859.1
• Coordinates: 1740899..1741150 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDX52_RS08330 (LDX52_08325)	1738425..1739354	-	930	WP_267271219.1	cytochrome C5	-
LDX52_RS08335 (LDX52_08330)	1739504..1740742	+	1239	WP_199283089.1	MFS transporter	-
LDX52_RS08340 (LDX52_08335)	1740899..1741150	+	252	WP_109918859.1	hypothetical protein	Antitoxin
LDX52_RS08345 (LDX52_08340)	1741144..1741476	+	333	WP_267271220.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LDX52_RS08350	1741563..1741694	+	132	WP_255409548.1	hypothetical protein	-
LDX52_RS08355 (LDX52_08345)	1741759..1742661	-	903	WP_267271221.1	LysR family transcriptional regulator	-
LDX52_RS08360 (LDX52_08350)	1742767..1744008	+	1242	WP_267271222.1	MFS transporter	-
LDX52_RS08365 (LDX52_08355)	1744013..1745452	+	1440	WP_267271223.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12758.84 Da
• Isoelectric Point: 8.3342

>T218483 WP_267271220.1 NZ_CP084384:1741144-1741476 [Lactobacillus helsingborgensis]
MVIKQGSILLMDLSPKQGHEQSGKQPYLYLSYNGVEKYAHIAVFAPISTTKRRYPLYQELPAVSQTKGVVMLDQLVTIDY
HNQPFHYLEQLPDDYMEKILKIVKVVFQKD

Nucleotide

• Length: 333 bp

>T218483 NZ_CP115992:2491410-2491526 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGCATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9591.75 Da
• Isoelectric Point: 4.4992

>AT218483 WP_109918859.1 NZ_CP084384:1740899-1741150 [Lactobacillus helsingborgensis]
MIRATISKWGNSKGIRLNQAVLQALNATIGDQLNFKIEDNKVIMSKQDDSPDLTFEELFKDYSGEEFDTEIKLPDERKGN
ERW

Nucleotide

• Length: 252 bp

>AT218483 NZ_CP115992:c2491642-2491437 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATGC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC