Detailed information of TA system TA218482

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 39395..41179
• Replicon: plasmid pLG50
• Accession: NZ_CP084378
• Organism: Lactococcus garvieae strain Lg-Granada

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: LA387_RS11055
• Protein ID: WP_107106298.1
• Coordinates: 39395..40327 (-)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: LA387_RS11060
• Protein ID: WP_107106297.1
• Coordinates: 40343..41179 (-)
• Length: 279 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA387_RS11035	35410..36126	+	717	WP_225667050.1	hypothetical protein	-
LA387_RS11170	36294..36419	+	126	WP_263867710.1	hypothetical protein	-
LA387_RS11040	36544..37308	+	765	WP_225667051.1	abortive infection protein	-
LA387_RS11045	37442..38392	-	951	WP_225667052.1	hypothetical protein	-
LA387_RS11050	38406..38957	-	552	WP_225667053.1	recombinase family protein	-
LA387_RS11055	39395..40327	-	933	WP_107106298.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LA387_RS11060	40343..41179	-	837	WP_107106297.1	hypothetical protein	Antitoxin
LA387_RS11065	42119..42259	+	141	WP_107106296.1	type I toxin-antitoxin system Fst family toxin	-
LA387_RS11070	42420..43598	-	1179	WP_225667054.1	protein kinase family protein	-
LA387_RS11075	43686..44243	-	558	WP_107106295.1	recombinase family protein	-
LA387_RS11080	44643..44828	+	186	WP_225667055.1	hypothetical protein	-
LA387_RS11085	44860..45159	+	300	WP_225667057.1	hypothetical protein	-
LA387_RS11090	45253..45465	+	213	WP_165719065.1	garvicin Q family class II bacteriocin	-
LA387_RS11095	45489..45803	+	315	WP_032495339.1	bacteriocin immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50557	50557
	-	flank	IS/Tn	-	-	38406..38957	551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (30-234)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 36228.16 Da
• Isoelectric Point: 5.3574

>T218482 WP_107106298.1 NZ_CP084378:c40327-39395 [Lactococcus garvieae]
MKMTRLRDLIASQPGITSADNYQIRYAIDRFLARLVKSKYKENFVIKGGFLQGVIYNLDQRATRDLDASVRDMKANYTAI
EKAVLEICSIHLNDNVSFKLRSLESSQEGRIYSGFRAKLTMSFLEERTKINFDLDIGVGDEITPRAQKIEFPLLFSEEKG
KTETIEIFGYPRQTSQAEKLETILVRGLSNSRMKDFYDMHLFYNDLESISVHQSCEAFVKTWEFRHPETEINEELFLDWF
DLLEDISSNKKFLNDYWPNYTKDKPFASPLSFKDIVQEIIELLKSIQEEYLTNKGEKYARDKSSDTKEKL

Nucleotide

• Length: 933 bp

>T218482 NZ_CP115992:c2337311-2337216 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 279 a.a.
• Molecular weight: 32659.68 Da
• Isoelectric Point: 9.0976

>AT218482 WP_107106297.1 NZ_CP084378:c41179-40343 [Lactococcus garvieae]
MDIYKISNINLDEQNKKNLKRVAENMLSTFDEEQLQSIIKVMMTPFYQNELSSYLIEKTLPAIGSEEFNFLLLANKYNGH
IIRKIVKEEGISDYYLRKFVEKYSLKELIKGSYIFPNKPVDYLFVLQQRYKITAVSHETALYMQELIDYIPQVITISIPE
KYNADLIYNSFVQNSFLNNELNFLKKLGDNRITFVKNKPIPPSQIIELADENGLPLRVTSAERSVVDILKSSYKADEEVK
EQAVNSFIRRFPERIARLRRIAKNQNSLKELENYLLRY

Nucleotide

• Length: 837 bp

>AT218482 NZ_CP115992:2337117-2337181 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT