Detailed information of TA system TA218481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1609283..1609847
• Replicon: chromosome
• Accession: NZ_CP084357
• Organism: Streptomyces sp. WA6-1-16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LEL86_RS07290
• Protein ID: WP_093438178.1
• Coordinates: 1609500..1609847 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: N0CUB8
• Locus tag: LEL86_RS07285
• Protein ID: WP_015608544.1
• Coordinates: 1609283..1609507 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEL86_RS07270 (LEL86_07270)	1604618..1605772	+	1155	WP_032762146.1	allantoicase	-
LEL86_RS07275 (LEL86_07275)	1605884..1606444	-	561	WP_225680378.1	hypothetical protein	-
LEL86_RS07280 (LEL86_07280)	1606603..1609179	-	2577	WP_225680379.1	aminopeptidase N	-
LEL86_RS07285 (LEL86_07285)	1609283..1609507	+	225	WP_015608544.1	ribbon-helix-helix domain-containing protein	Antitoxin
LEL86_RS07290 (LEL86_07290)	1609500..1609847	+	348	WP_093438178.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LEL86_RS07295 (LEL86_07295)	1609929..1610957	-	1029	WP_032762131.1	aspartate-semialdehyde dehydrogenase	-
LEL86_RS07300 (LEL86_07300)	1611214..1614522	-	3309	WP_176741689.1	S8 family serine peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12699.66 Da
• Isoelectric Point: 11.5710

>T218481 WP_093438178.1 NZ_CP084357:1609500-1609847 [Streptomyces sp. WA6-1-16]
MRRGDIHLVDLEPARGSEANKVRPAVIVSNNAANRSAEVSGRGVVAVVPLTSNTSRILSFQVLLTAEESRLPQNSKVQCE
QIRAVSPDRILKRLGEVPRQRMAEIDKALRRHLAL

Nucleotide

• Length: 348 bp

>T218481 NZ_CP115992:c2060380-2060198 [Enterococcus faecalis]
ATGCCAATGACACAAAAACAAATGGTCAAATTGCTAAAAAAGCACGGTTGGTTACCAACCGATGGTGGAAAAGGCTCCCA
TATAAAAATGGAGATGTCTGGCAAAAGACCCATCATTATCCCACACGGAGAATTGAAAAAAGGAACCGAAATAGGGATTT
TAAAAGAGGCAGGGCTGAAATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8061.79 Da
• Isoelectric Point: 3.7720

>AT218481 WP_015608544.1 NZ_CP084357:1609283-1609507 [Streptomyces sp. WA6-1-16]
MKISVSLPQEDVAFVDEYAAKTDADSRSAVIHAAIELLRTAGLEAEYTEAFQEWDASEDAALWDRTVADGLTDA

Nucleotide

• Length: 225 bp

>AT218481 NZ_CP115992:c2060164-2059727 [Enterococcus faecalis]
ATGCTAATTAGTTATCCTGCATGTTTCTACAGTGTCGAGAATGGTTACTATGTTTATTTCCCAGATATAGGTGGTTCTGG
TACGCAAGGAGATACAATTCCTGATGCAATTGCGATGGCTTCCGATTATTTAGGTATGATGTTATCTGATGATATTGAAC
ATCAAAGAACAATCCACCAGCCATCTCCTATCAATACGTTGTCATTAGAAGATAATAATCCCTTTAGAGATGACGATGAC
TTTAATTTTAACCTTGCAGACTCGTTTGTTTCTATGGTAAATGTAGAACTAAATGATTATTTAGGAACAAATACGTTAGT
CAAAAAAACCGTCACCATTCCCAAGTGGTCTGATGAACTCGGGAAAAAAAATAAATTGAATTTTTCTAAAACCTTGACCG
ATGCCATCGTTGAAAAGTCGCTACATGTTTTAAAGTAG

Structures

Antitoxin

• AlphaFold DB: N0CUB8