Detailed information of TA system TA218437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2292390..2293036
• Replicon: chromosome
• Accession: NZ_CP084336
• Organism: Yersinia pestis strain R

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G5L3F2
• Locus tag: LDH64_RS10460
• Protein ID: WP_002214976.1
• Coordinates: 2292390..2292572 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0U1R391
• Locus tag: LDH64_RS10465
• Protein ID: WP_002211129.1
• Coordinates: 2292617..2293036 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH64_RS10425 (LDH64_10415)	2287776..2288246	+	471	WP_002227961.1	flagellar basal body-associated protein FliL	-
LDH64_RS10430 (LDH64_10420)	2288252..2289253	+	1002	WP_002211135.1	flagellar motor switch protein FliM	-
LDH64_RS10435 (LDH64_10425)	2289250..2289666	+	417	WP_002211134.1	flagellar motor switch protein FliN	-
LDH64_RS10440 (LDH64_10430)	2289666..2290202	+	537	WP_002211133.1	flagellar biosynthetic protein FliO	-
LDH64_RS10445 (LDH64_10435)	2290283..2291023	+	741	WP_002220494.1	flagellar type III secretion system pore protein FliP	-
LDH64_RS10450 (LDH64_10440)	2291129..2291398	+	270	WP_002211131.1	flagellar biosynthesis protein FliQ	-
LDH64_RS10455 (LDH64_10445)	2291401..2292183	+	783	WP_002211130.1	flagellar biosynthetic protein FliR	-
LDH64_RS10460 (LDH64_10450)	2292390..2292572	+	183	WP_002214976.1	type II toxin-antitoxin system HicA family toxin	Toxin
LDH64_RS10465 (LDH64_10455)	2292617..2293036	+	420	WP_002211129.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LDH64_RS10470 (LDH64_10460)	2293294..2294253	-	960	WP_002211128.1	sugar kinase	-
LDH64_RS10475 (LDH64_10465)	2294302..2295324	-	1023	WP_002214974.1	ABC transporter permease	-
LDH64_RS10480 (LDH64_10470)	2295327..2296904	-	1578	WP_002211126.1	sugar ABC transporter ATP-binding protein	-
LDH64_RS10485 (LDH64_10475)	2296970..2297965	-	996	WP_002211125.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6783.99 Da
• Isoelectric Point: 10.8877

>T218437 WP_002214976.1 NZ_CP084336:2292390-2292572 [Yersinia pestis]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Nucleotide

• Length: 183 bp

>T218437 NZ_CP115953:c60891-60586 [Ralstonia solanacearum CFBP2957]
ATGACGATGGCCCAACGCACCCTCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAGGCGGACATGGCCGC
CTATCTCAACGCCTGCCTGGAAGAGGCCGGGGACGACCCGGCGTTCATCACGCACGCGCTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACCCGGCTCGCCCGCGAGACCGGGATCACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAACCCA
AGCTTTGCGACCATGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15549.42 Da
• Isoelectric Point: 4.7731

>AT218437 WP_002211129.1 NZ_CP084336:2292617-2293036 [Yersinia pestis]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Nucleotide

• Length: 420 bp

>AT218437 NZ_CP115953:c61205-60888 [Ralstonia solanacearum CFBP2957]
ATGCTGACGATCCGCACGACCGAACTCTTTGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGTGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCGGGCGACATGAAAGCGGTACGTGACGGCATCCGCGAACTGCGGATCG
ATCATGGGCCGGGCTACCGCTTGTACTTCGTGCAGCACGGCGTCGTCCTGATCGTACTGCTCTGCGGCGGCGCCAAGTCG
ACGCAGGAAGCCGATAGCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACGTGGACGACGTGGAATGA

Structures

Toxin

• AlphaFold DB: A0A3G5L3F2

Antitoxin

• AlphaFold DB: A0A0U1R391