Detailed information of TA system TA218432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5805577..5806163
• Replicon: chromosome
• Accession: NZ_CP084321
• Organism: Pseudomonas aeruginosa strain HS18-89

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LEL82_RS27180
• Protein ID: WP_003120987.1
• Coordinates: 5805864..5806163 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LEL82_RS27175
• Protein ID: WP_003448662.1
• Coordinates: 5805577..5805867 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEL82_RS27155 (LEL82_27120)	5801159..5803048	+	1890	WP_196994395.1	hypothetical protein	-
LEL82_RS27160 (LEL82_27125)	5803045..5805021	+	1977	WP_274447201.1	DEAD/DEAH box helicase	-
LEL82_RS27165	5805031..5805165	+	135	WP_255253641.1	hypothetical protein	-
LEL82_RS27170 (LEL82_27130)	5805162..5805506	+	345	WP_016851612.1	hypothetical protein	-
LEL82_RS27175 (LEL82_27135)	5805577..5805867	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LEL82_RS27180 (LEL82_27140)	5805864..5806163	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LEL82_RS27185 (LEL82_27145)	5806365..5807489	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
LEL82_RS27190 (LEL82_27150)	5807489..5809198	+	1710	WP_223723594.1	PilN family type IVB pilus formation outer membrane protein	-
LEL82_RS27195 (LEL82_27155)	5809202..5810527	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
LEL82_RS27200 (LEL82_27160)	5810517..5811050	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5777547..5866542	88995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T218432 WP_003120987.1 NZ_CP084321:c5806163-5805864 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T218432 NZ_CP115947:110719-111048 [Ralstonia solanacearum MolK2]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCAGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGACGATCTTTCCAGTGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT218432 WP_003448662.1 NZ_CP084321:c5805867-5805577 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT218432 NZ_CP115947:111045-111356 [Ralstonia solanacearum MolK2]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGACCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCGCGTCGGCTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G8CP73