Detailed information of TA system TA218431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4833400..4834040
• Replicon: chromosome
• Accession: NZ_CP084321
• Organism: Pseudomonas aeruginosa strain HS18-89

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LEL82_RS22605
• Protein ID: WP_003134109.1
• Coordinates: 4833400..4833810 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LEL82_RS22610
• Protein ID: WP_031634724.1
• Coordinates: 4833810..4834040 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEL82_RS22565 (LEL82_22535)	4828433..4829758	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
LEL82_RS22570 (LEL82_22540)	4829755..4830267	+	513	WP_274447187.1	hypothetical protein	-
LEL82_RS22575 (LEL82_22545)	4830424..4831152	+	729	WP_034014909.1	TIGR03761 family integrating conjugative element protein	-
LEL82_RS22580 (LEL82_22550)	4831158..4831691	+	534	WP_274447188.1	DUF3158 family protein	-
LEL82_RS22585 (LEL82_22555)	4831705..4832193	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
LEL82_RS22590 (LEL82_22560)	4832692..4832889	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
LEL82_RS22595 (LEL82_22565)	4832955..4833173	-	219	WP_023083218.1	hypothetical protein	-
LEL82_RS22600 (LEL82_22570)	4833223..4833384	-	162	WP_191519260.1	hypothetical protein	-
LEL82_RS22605 (LEL82_22575)	4833400..4833810	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LEL82_RS22610 (LEL82_22580)	4833810..4834040	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LEL82_RS22615 (LEL82_22585)	4834358..4836283	+	1926	WP_191519261.1	type I DNA topoisomerase	-
LEL82_RS22620 (LEL82_22590)	4836297..4837043	+	747	WP_231405939.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4814934..4943211	128277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T218431 WP_003134109.1 NZ_CP084321:c4833810-4833400 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T218431 NZ_CP115946:c2760266-2759853 [Ralstonia solanacearum MolK2]
ATGCCCGCGGCTGAGGCGTTTTTCGACAGCAACATCGTGCTGTACCTGCTGTCGGCCGATGCCGCCAAGGCCGATGCGGC
GGAAGCGCTACTGATGGCCGGCGGCGTCGTCAGCGTGCAGGTGCTCAACGAAACGACGCACGTGATGCGCCGCAAGCTCG
CCATGCCGTGGCACGCGATCGAAGCGGTGCAGGAAGCCGTACGCGCGCAATGCCGGGTCGAGCCGCTGACGGTCGAAACC
CATGATCTCGGGCGGCATCTTGCGCAGCGCTATGGATTGTCGGTCTATGACGCGCTGATCGTCGCCGCGGCGTCGCTGGC
CGGTTGCCGCGTCCTGTATTCCGAGGACATGCAGCACGGCCTGGTGATCGAGCAGCGTCTGCGCGTCGTCAATCCTTTTG
TCGCGTCGGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT218431 WP_031634724.1 NZ_CP084321:c4834040-4833810 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT218431 NZ_CP115946:c2760486-2760253 [Ralstonia solanacearum MolK2]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCCATCCGGCTGCCCGCAGCCGTTGTGGAGGCGCTTGGACTGCACGAAGG
CGACGACGTCGAGGTGGTGGTGGCGGGCGAACGCTCGTTTGGTGTGCGCCGCAAGCGTTCCGCGCGTGAGTTGTTTGAGC
GGCTCCACCAATACCGCGGCAAGCTGCCCGCCGACTTCATCTTCGAGCGCGACGACGCCAATGCCCGCGGCTGA