Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 101285..101918 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag BCG_RS00515 Protein ID WP_003400580.1
Coordinates 101517..101918 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7U4BR76
Locus tag BCG_RS00510 Protein ID WP_011799070.1
Coordinates 101285..101524 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS00500 96582..98021 + 1440 WP_003901784.1 FAD-binding oxidoreductase -
BCG_RS20515 98077..98238 + 162 WP_003899796.1 hypothetical protein -
BCG_RS00505 98279..101218 + 2940 WP_003901785.1 UPF0182 family protein -
BCG_RS00510 101285..101524 + 240 WP_011799070.1 hypothetical protein Antitoxin
BCG_RS00515 101517..101918 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
BCG_RS00520 101970..104207 - 2238 WP_003400583.1 NADP-dependent isocitrate dehydrogenase -
BCG_RS00525 104325..104894 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator -
BCG_RS00530 104997..105908 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T21843 WP_003400580.1 NC_008769:101517-101918 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T21843 NC_008769:101517-101918 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8745.89 Da        Isoelectric Point: 8.5960

>AT21843 WP_011799070.1 NC_008769:101285-101524 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEVIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT21843 NC_008769:101285-101524 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGTAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BR76

References