Detailed information of TA system TA218424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1216063..1216660
• Replicon: chromosome
• Accession: NZ_CP084313
• Organism: Bifidobacterium pseudolongum strain 46-E-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LDX61_RS04920
• Protein ID: WP_080699166.1
• Coordinates: 1216063..1216365 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N3QV32
• Locus tag: LDX61_RS04925
• Protein ID: WP_026643099.1
• Coordinates: 1216367..1216660 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDX61_RS04890 (LDX61_04890)	1211899..1212717	+	819	WP_225545080.1	dihydroorotate dehydrogenase electron transfer subunit	-
LDX61_RS04895 (LDX61_04895)	1212702..1213691	+	990	WP_129989983.1	dihydroorotate dehydrogenase	-
LDX61_RS04900 (LDX61_04900)	1213732..1214427	+	696	WP_039173251.1	orotate phosphoribosyltransferase	-
LDX61_RS04905 (LDX61_04905)	1214461..1215279	+	819	WP_080699168.1	serpin family protein	-
LDX61_RS04910 (LDX61_04910)	1215515..1215751	+	237	WP_225531405.1	hypothetical protein	-
LDX61_RS04915 (LDX61_04915)	1215764..1215952	+	189	WP_225532136.1	GNAT family N-acetyltransferase	-
LDX61_RS04920 (LDX61_04920)	1216063..1216365	+	303	WP_080699166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDX61_RS04925 (LDX61_04925)	1216367..1216660	+	294	WP_026643099.1	putative addiction module antidote protein	Antitoxin
LDX61_RS04930 (LDX61_04930)	1216782..1217126	-	345	WP_225531406.1	helix-turn-helix domain-containing protein	-
LDX61_RS04935 (LDX61_04935)	1217293..1218222	-	930	WP_026643097.1	LysR family transcriptional regulator	-
LDX61_RS04940 (LDX61_04940)	1218256..1218897	-	642	WP_225531407.1	HAD family phosphatase	-
LDX61_RS04945 (LDX61_04945)	1219092..1220840	-	1749	WP_225531408.1	FAD-dependent oxidoreductase	-
LDX61_RS04950 (LDX61_04950)	1220867..1221430	-	564	WP_033488853.1	alkyl hydroperoxide reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11494.14 Da
• Isoelectric Point: 9.8170

>T218424 WP_080699166.1 NZ_CP084313:1216063-1216365 [Bifidobacterium pseudolongum]
MEIRTTEQFDKWFRKLRDRKAKAKISARLRQCEISDHITGDHHGIGGGISEMRFHFGPGYRIYYTVDNDVLVILLIGSGK
DDQSRGIAEARKLLALYRGE

Nucleotide

• Length: 303 bp

>T218424 NZ_CP115945:c70017-69712 [Ralstonia syzygii R24]
ATGATGATGGCCCAACACACCATAAAAACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTATCTCGACGCCTGCCTGGAGGAAGCCGGGGATGACCCCGCATTCATCACGCACGCGCTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACGCAGATCGCCCGCGAGACCGGGATGACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAATCCG
AGCTTTGCAACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGTTGCACGCCGTGCCGGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10542.01 Da
• Isoelectric Point: 4.5837

>AT218424 WP_026643099.1 NZ_CP084313:1216367-1216660 [Bifidobacterium pseudolongum]
MTLKTTKWDVTEYLDNEEDIAEYLNAVIELDDPALLQAAIGDVAKAQGMTKIAKDAGLGRESLYKSLSAQGNPSFTTILK
VLHALGTRISIQPEHAI

Nucleotide

• Length: 294 bp

>AT218424 NZ_CP115945:c70322-70014 [Ralstonia syzygii R24]
ATGCTGACAATCCGCACGACTGAAATCTTCGACGACTGGTTTTGCAGTCTCCGCGACAAGGCCGCGCAACGTCGCGTCCA
GGTGCGCATCGATCGCCTGCAGATGGGCAATCCAGGCGATGTGAAAGCTGTACGTGAAGGCATCCGTGAGATGCGGATCG
ATCATGGCCCGGGCTATCGCGTGTATTTTGCACAGCGTGGCGTGATCCTGATCGTCCTGCTGTGCGGCGGTGACAAGTCG
ACGCAGGAAGCCGATGTCCGCCGCGCAATCGACCTGTCCCGCCGTCTTGACGTGGACAACATGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2N3QV32