Detailed information of TA system TA218423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1216098..1216695
• Replicon: chromosome
• Accession: NZ_CP084312
• Organism: Bifidobacterium pseudolongum strain 46-D-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LDH93_RS04925
• Protein ID: WP_080699166.1
• Coordinates: 1216098..1216400 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N3QV32
• Locus tag: LDH93_RS04930
• Protein ID: WP_026643099.1
• Coordinates: 1216402..1216695 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDH93_RS04895 (LDH93_04895)	1211934..1212752	+	819	WP_225542988.1	dihydroorotate dehydrogenase electron transfer subunit	-
LDH93_RS04900 (LDH93_04900)	1212737..1213726	+	990	WP_129989983.1	dihydroorotate dehydrogenase	-
LDH93_RS04905 (LDH93_04905)	1213767..1214462	+	696	WP_039173251.1	orotate phosphoribosyltransferase	-
LDH93_RS04910 (LDH93_04910)	1214496..1215314	+	819	WP_080699168.1	serpin family protein	-
LDH93_RS04915 (LDH93_04915)	1215550..1215786	+	237	WP_225531405.1	hypothetical protein	-
LDH93_RS04920 (LDH93_04920)	1215799..1215987	+	189	WP_225532136.1	GNAT family N-acetyltransferase	-
LDH93_RS04925 (LDH93_04925)	1216098..1216400	+	303	WP_080699166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDH93_RS04930 (LDH93_04930)	1216402..1216695	+	294	WP_026643099.1	putative addiction module antidote protein	Antitoxin
LDH93_RS04935 (LDH93_04935)	1216817..1217161	-	345	WP_225531406.1	helix-turn-helix domain-containing protein	-
LDH93_RS04940 (LDH93_04940)	1217328..1218257	-	930	WP_026643097.1	LysR family transcriptional regulator	-
LDH93_RS04945 (LDH93_04945)	1218291..1218932	-	642	WP_225531407.1	HAD family phosphatase	-
LDH93_RS04950 (LDH93_04950)	1219127..1220875	-	1749	WP_225531408.1	FAD-dependent oxidoreductase	-
LDH93_RS04955 (LDH93_04955)	1220902..1221465	-	564	WP_033488853.1	alkyl hydroperoxide reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11494.14 Da
• Isoelectric Point: 9.8170

>T218423 WP_080699166.1 NZ_CP084312:1216098-1216400 [Bifidobacterium pseudolongum]
MEIRTTEQFDKWFRKLRDRKAKAKISARLRQCEISDHITGDHHGIGGGISEMRFHFGPGYRIYYTVDNDVLVILLIGSGK
DDQSRGIAEARKLLALYRGE

Nucleotide

• Length: 303 bp

>T218423 NZ_CP115944:3453358-3453771 [Ralstonia syzygii R24]
TTGAGTTACCTGATCGACACCAATGTCCTGTCCGAATTGCGTCGCAAAGCGCCCGATGCGCGCGTGGTGGCCTGGATGCA
GGACCGCCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAGATTCGCAAAGGTATCGAGCGGCTGGACGACG
CGGTACGCCGCCAGAACCTGATTGACTGGCTCGAAGTCGAACTGCCGAACTACTTTCTCGGCCGATTGCTCGACATCGAT
GCACATACTGCGGACCGCTGGGGCCGGCTGATGTCCTCGGCCGGCCGCCCCCTGCCCGCCATCGACGGCCTGCTGGCCGC
GACCGCCCTGCAACACGACCTCACGCTGGTCACGCGCAACATCAAGGATTTCGCGGGGCTGGACGTCCAGCTCATCAACC
CATGGGAGGCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10542.01 Da
• Isoelectric Point: 4.5837

>AT218423 WP_026643099.1 NZ_CP084312:1216402-1216695 [Bifidobacterium pseudolongum]
MTLKTTKWDVTEYLDNEEDIAEYLNAVIELDDPALLQAAIGDVAKAQGMTKIAKDAGLGRESLYKSLSAQGNPSFTTILK
VLHALGTRISIQPEHAI

Nucleotide

• Length: 294 bp

>AT218423 NZ_CP115944:3453110-3453361 [Ralstonia syzygii R24]
ATGCAAAGCTGGCAAATGCAGGCCGCCAAGGCGCGGTTTTCCGATGTCGTGAAGCGGGCAGCAGACGACGGCCCGCAGGA
AATCACCGTGCATGGCCGCCCGGTGGCGGTAGTGATCTCGCGCGCACTGTTCGATCGCTTGAGCGGCAGCGGTGAGTCGC
TGGTCAGCTTCATGCGCCAATCCCCGCTGGCCGATCAGGACGATGTCGTTTTTGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N3QV32