Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 79329..79917 | Replicon | plasmid pXMC5X702-tetX-145k |
| Accession | NZ_CP084302 | ||
| Organism | Acinetobacter pseudolwoffii strain XMC5X702 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | A0A5N5XUQ1 |
| Locus tag | LDO52_RS15585 | Protein ID | WP_047930174.1 |
| Coordinates | 79329..79616 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | A0A0J0ZQ33 |
| Locus tag | LDO52_RS15590 | Protein ID | WP_047930176.1 |
| Coordinates | 79603..79917 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDO52_RS15565 (LDO52_15570) | 75186..75617 | + | 432 | WP_225554395.1 | hypothetical protein | - |
| LDO52_RS15570 (LDO52_15575) | 76248..76859 | - | 612 | WP_004811709.1 | recombinase family protein | - |
| LDO52_RS15575 (LDO52_15580) | 77074..77601 | + | 528 | WP_000537573.1 | plasmid pRiA4b ORF-3 family protein | - |
| LDO52_RS15580 (LDO52_15585) | 77733..79067 | + | 1335 | WP_001986494.1 | ISNCY-like element ISAba32 family transposase | - |
| LDO52_RS15585 (LDO52_15590) | 79329..79616 | + | 288 | WP_047930174.1 | BrnT family toxin | Toxin |
| LDO52_RS15590 (LDO52_15595) | 79603..79917 | + | 315 | WP_047930176.1 | BrnA antitoxin family protein | Antitoxin |
| LDO52_RS15595 (LDO52_15600) | 80000..80335 | + | 336 | WP_000689753.1 | hypothetical protein | - |
| LDO52_RS15600 (LDO52_15605) | 80385..80564 | + | 180 | WP_001231652.1 | hypothetical protein | - |
| LDO52_RS15605 (LDO52_15610) | 80561..80947 | + | 387 | WP_000053135.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| LDO52_RS15610 (LDO52_15615) | 80949..81500 | - | 552 | WP_000755487.1 | macro domain-containing protein | - |
| LDO52_RS15615 (LDO52_15620) | 81506..82189 | - | 684 | WP_000415733.1 | hypothetical protein | - |
| LDO52_RS15620 (LDO52_15625) | 82266..82721 | - | 456 | WP_000181347.1 | metallophosphoesterase | - |
| LDO52_RS15625 (LDO52_15630) | 82894..83238 | - | 345 | WP_005102094.1 | helix-turn-helix transcriptional regulator | - |
| LDO52_RS15630 (LDO52_15635) | 83802..84152 | + | 351 | WP_000556949.1 | hypothetical protein | - |
| LDO52_RS15635 (LDO52_15640) | 84170..84787 | - | 618 | WP_000831517.1 | plasmid mobilization relaxosome protein MobC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | msr(E) / mph(E) / floR / blaTEM-2 / aac(3)-IIa / aph(6)-Id / sul2 / dfrA36 / tet(X3) / tet(X6) / lnu(G) | - | 1..145524 | 145524 | |
| - | inside | IScluster/Tn | - | - | 59747..79067 | 19320 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11276.65 Da Isoelectric Point: 5.6153
>T218420 WP_047930174.1 NZ_CP084302:79329-79616 [Acinetobacter pseudolwoffii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG
Download Length: 288 bp
>T218420 NZ_CP115944:c1243146-1242883 [Ralstonia syzygii R24]
ATGAAAGCCAAACACAAAAAGACGTTGGACCTGATCTTTTCCCGACCGACCCCGGCGGGGGTCAAATGGAACGACTTGGT
CGGTCTGCTGGTTGAGCTTGGCGCCGAGATCACCGAGCGGGAAGGCTCACGGGTTGCTGTGTTCCTGTTCAATCAGGTGA
AGGTGATGCACAGGCCGCACCCGTCCCCAGACGTGGACAAAGGCGCGGTCGCCTCGATCCGAAAGTGGCTCGAAGACAAC
GCAACGGAGTGGAACAATGAATAA
ATGAAAGCCAAACACAAAAAGACGTTGGACCTGATCTTTTCCCGACCGACCCCGGCGGGGGTCAAATGGAACGACTTGGT
CGGTCTGCTGGTTGAGCTTGGCGCCGAGATCACCGAGCGGGAAGGCTCACGGGTTGCTGTGTTCCTGTTCAATCAGGTGA
AGGTGATGCACAGGCCGCACCCGTCCCCAGACGTGGACAAAGGCGCGGTCGCCTCGATCCGAAAGTGGCTCGAAGACAAC
GCAACGGAGTGGAACAATGAATAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12285.00 Da Isoelectric Point: 9.4646
>AT218420 WP_047930176.1 NZ_CP084302:79603-79917 [Acinetobacter pseudolwoffii]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKKHQ
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKKHQ
Download Length: 315 bp
>AT218420 NZ_CP115944:c1242890-1242552 [Ralstonia syzygii R24]
ATGAATAACGTCATGATCATCGGCGGCGAAAAAGCCGTCATTTCCTTCGATCCGGAACTGGAAATGTTTCGCGGCGAATT
CATCGGCCTGAATGGCGGTGCGGATTTTTACGCCAGTGATGTGGCTGGCCTGCACCGTGAAGGCGAGCTGTCGCTTCACA
CCTTCATGGAGGAGTGCGTGCGCCGCGGCGTCGAACCGCGCCGCAGTTTCTCTGGCAAGTTCGTGTTACGCACGTCGGAG
AAGACACATGAAGCGGCCGCCATCGCCGCCGCCGCACATGGTGTGAGCCTGAACCAATGGGTGTCCGACGTGATCGAGCA
GGCCGCCATCCCAGCTTAA
ATGAATAACGTCATGATCATCGGCGGCGAAAAAGCCGTCATTTCCTTCGATCCGGAACTGGAAATGTTTCGCGGCGAATT
CATCGGCCTGAATGGCGGTGCGGATTTTTACGCCAGTGATGTGGCTGGCCTGCACCGTGAAGGCGAGCTGTCGCTTCACA
CCTTCATGGAGGAGTGCGTGCGCCGCGGCGTCGAACCGCGCCGCAGTTTCTCTGGCAAGTTCGTGTTACGCACGTCGGAG
AAGACACATGAAGCGGCCGCCATCGCCGCCGCCGCACATGGTGTGAGCCTGAACCAATGGGTGTCCGACGTGATCGAGCA
GGCCGCCATCCCAGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5N5XUQ1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J0ZQ33 |