Detailed information of TA system TA218419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 69972..70500
• Replicon: plasmid pXMC5X702-tetX-145k
• Accession: NZ_CP084302
• Organism: Acinetobacter pseudolwoffii strain XMC5X702

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9N871
• Locus tag: LDO52_RS15535
• Protein ID: WP_000221358.1
• Coordinates: 70207..70500 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9NWT9
• Locus tag: LDO52_RS15530
• Protein ID: WP_000246755.1
• Coordinates: 69972..70217 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO52_RS15515 (LDO52_15515)	65010..65999	-	990	WP_005093385.1	copper resistance protein B	-
LDO52_RS15520 (LDO52_15520)	65986..67842	-	1857	WP_159138998.1	copper resistance system multicopper oxidase	-
LDO52_RS15525 (LDO52_15530)	68523..69857	+	1335	WP_107009803.1	ISNCY family transposase	-
LDO52_RS15530 (LDO52_15535)	69972..70217	+	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LDO52_RS15535 (LDO52_15540)	70207..70500	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDO52_RS15540 (LDO52_15545)	70583..71917	-	1335	WP_148334750.1	ISNCY-like element ISAba32 family transposase	-
LDO52_RS15545 (LDO52_15550)	72072..72266	+	195	WP_004811689.1	hypothetical protein	-
LDO52_RS15550 (LDO52_15555)	72695..73123	+	429	WP_121775635.1	transposase	-
LDO52_RS15555 (LDO52_15560)	73120..73455	+	336	WP_121775636.1	IS66 family insertion sequence element accessory protein TnpB	-
LDO52_RS15560 (LDO52_15565)	73530..75176	+	1647	WP_121775637.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / floR / blaTEM-2 / aac(3)-IIa / aph(6)-Id / sul2 / dfrA36 / tet(X3) / tet(X6) / lnu(G)	-	1..145524	145524
	-	inside	IScluster/Tn	-	-	59747..79067	19320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11292.52 Da
• Isoelectric Point: 10.4753

>T218419 WP_000221358.1 NZ_CP084302:70207-70500 [Acinetobacter pseudolwoffii]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T218419 NZ_CP115942:c67047-66739 [Ralstonia syzygii]
ATGCTGACAATCCGCACGACTGAAATCTTCGACGACTGGTTTTACAGTCTCCGCGACAAGGCCGCGCAACGCCGCGTCCA
GGTGCGCATCGATCGCCTGCAGATGGGCAATCCAGGCGATGTGAAAACCGTACGTGAAGGCATCCGTGAGATGCGGATCG
ATCATGGCCCGGGCTATCGCGTGTATGTTGCGCAGCGTGGCGTGGTCCTGATCGTCCTGCTGTGCGGCGGTGACAAGTCG
ACGCAGGAAGCCGATGTCCGCCGCGCAATCGACCTGTCCCGCCGTCTTGACGTGGACAACATGGAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9249.64 Da
• Isoelectric Point: 4.3142

>AT218419 WP_000246755.1 NZ_CP084302:69972-70217 [Acinetobacter pseudolwoffii]
MATVNFRIDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT218419 NZ_CP115942:c66742-66437 [Ralstonia syzygii]
ATGATGATGGCCCAACGCACCATAAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTATCTCGACGCCTGCCTGGAGGAAGCCGGGGATGACCCCGCATTCATCACGCACGCGCTGGGCGTGGTCGCGCGGGCGC
GCGGCATGACGCAGATCGCCCGCGAGACCGGGATGACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6A1RKP4

Antitoxin

• AlphaFold DB: A0A6A1RJ80